PLC-delta 3 Antibody [Alexa Fluor® 488]

• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

PLC-delta 3 Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human PLC‑δ3
  Lys148-Ala344
  Accession # Q8N3E9
• Specificity: Detects human and mouse PLC‑δ3 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

PLC-δ3 (phospholipase C-delta 3) is an 105 kDa member of the δ-type PLC isozyme family. It is expressed in corneal epithelium, platelets, cardiac and skeletal muscle. PLC-δ3 preferentially hydrolyzes membrane PIP2. This generates IP3, which induces intracellular Ca⁺⁺ release, and DAG, activating PKC. Human PLC-δ3 is 789 amino acids (aa) in length. It contains a plextrin homology domain that tethers the enzyme to the membrane (aa 63‑172), three EF-hand motifs that spatially orient the molecule (aa 182‑285), a catalytic region (aa 337‑644), and a C2 domain that secures the catalytic region to its substrate (aa 647‑752). There are multiple potential isoforms. There is an alternate start site at Met54, a 40 aa substitution for the C-terminal 248 aa, and a Leu substitution for aa 102‑109. Over aa 148‑344, human PLC-δ3 is 93% aa identical to mouse PLC-δ3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.