Human Plasminogen ELISA Kit (Colorimetric) Summary
| Standard Curve Range |
1 - 64 ng/ml |
| Sensitivity |
<2.01 ng/ml |
| Assay Type |
Competitive ELISA |
| Inter-Assay |
Mean 2.08% |
| Intra-Assay |
Mean 4.73% |
| Spike Recovery |
Serum - 100.14% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
PLG |
Applications/Dilutions
Packaging, Storage & Formulations
Kit Components
|
Components
|
- AV-HRP
- Dilution Buffers
- Incubation Buffer
- Microtiter Plate
- Protein Standard
- Secondary Antibody
- Stop Solution
- TMB Substrate
- Washing Buffer
|
Alternate Names for Human Plasminogen ELISA Kit (Colorimetric)
Background
Plasminogen, a 92kDa glycoprotein, is produced by the liver and is present in plasma and extracellular fluids. Plasminogen is the inactive precursor of plasmin, a potent serine protease involved in the dissolution of fibrin blood clots. Plasminogen can be converted into the active plasmin by plasminogen activators urokinase (uPA), tissue plasminogen activator (tPA), factor XII-dependent components. The plasmin system has been implicated in a variety of physiological and pathological processes such as fibrinolysis, tissue remodeling, cell migration, inflammation, and tumor invasion and metastasis. Hereditary defects of plasminogen are a predisposing risk factor for thromboembolic disease.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Plasminogen ELISA Kit (NBP2-61297). (Showing 1 - 2 of 2 FAQ).
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What is the difference between your Human Plasminogen ELISA kits NBP2-60521, NBP2-60515 and NBP2-61297? Also, have these been tested for reactivity against plasmin?
- Our Human Plasminogen ELISA kits NBP2-60521, NBP2-60515 and NBP2-61297 are all colorimeteric ELISA kits. However, these kits are different in reference to the suitable sample type, sensitivity, detection range etc. NBP2-61297 is a Competitive ELISA kit while the other two are Sandwich ELISA based. Also, NBP2-60521 is a mouse reactive kit while the other two are good for human samples.
-
What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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