Plasma Kallikrein/KLKB1 Antibody

Product Discontinued

Plasma Kallikrein/KLKB1 Antibody Summary

• Immunogen: Human Prekallikrein (PK) purified from plasma.
• Specificity: Specific for PK as demonstrated by immunoelectrophoresis and ELISA.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: KLKB1
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000
  • Immunodiffusion

Reactivity Notes

Cross reactivity: porcine, rabbit.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.4), 0.15M NaCl and 50% Glycerol
• Preservative: No Preservative
• Purity: Ammonium sulfate precipitation

Alternate Names for Plasma Kallikrein/KLKB1 Antibody

• EC 3.4.21
• EC 3.4.21.34
• Fletcher factor
• kallikrein B, plasma (Fletcher factor) 1
• kininogenin
• KLK3plasma kallikrein
• KLKB1
• plasma kallikrein heavy chain
• plasma kallikrein light chain
• Plasma Kallikrein
• Plasma Prekallikrein
• PPK

Background

Prekallikrein (PK), previously known as Fletcher Factor, is a single chain glycoprotein produced in the liver. The plasma concentration of PK is 50ug/ml (550nM), approximately 75% of which circulates in complex with high molecular weight kininogen (HK) and the remainder as free PK. Plasma PK is heterogeneous in both mass and charge due to variable degrees of glycosylation. Approximately 90% of plasma PK has an apparent molecular weight of 88kD as determined by SDS-PAGE and the remaining 10% has an apparent mass of 85kD. The catalytic site resides in the light chain. The heavy chain of PK contains four apple-domain structures similar to those found in FXI and these are required for binding of PK to HK. PK is the zymogen form of the enzyme kallikrein, which is involved in the proteolysis of kininogens with subsequent release of bradykinin, a potent vasodilator. PK participates in the contact phase of coagulation as a substrate for surface- bound activated factor XII (FXIIa) in the presence of the cofactor HK. As PK and factor XI (FXI) both circulate in complex with HK, both are localized to activating surfaces through their respective binding to HK. Limited proteolysis of PK by FXIIa generates kallikrein, a two-chain serine protease that initiates the reciprocal activation of PK and FXI. Kallikrein activity in plasma is regulated predominantly by C1-Inhibitor and alpha-2 macroglobulin, with relatively minor contributions by Protein C Inhibitor, alpha2antiplasmin, and antithrombin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KLKB1