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![Western Blot: Plasma Kallikrein/KLKB1 Antibody (JE31-29) [NBP3-32506] - Western blot analysis of Plasma Kallikrein/KLKB1 on human liver tissue lysates with Rabbit anti-Plasma Kallikrein/KLKB1 antibody (NBP3-32506) at 1/1,000 dilution. Lysates/proteins at 30 ug/Lane. Predicted band size: 71 kDa Observed band size: 71 kDa Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32506) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32506_rabbit-plasma-kallikrein-klkb1-mab-je31-29-278202416285248.jpg "Western Blot: Plasma Kallikrein/KLKB1 Antibody (JE31-29) [NBP3-32506] - Western blot analysis of Plasma Kallikrein/KLKB1 on human liver tissue lysates with Rabbit anti-Plasma Kallikrein/KLKB1 antibody (NBP3-32506) at 1/1,000 dilution. Lysates/proteins at 30 ug/Lane. Predicted band size: 71 kDa Observed band size: 71 kDa Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32506) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Plasma Kallikrein/KLKB1 Antibody (JE31-29) [NBP3-32506] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Plasma Kallikrein/KLKB1 antibody (NBP3-32506) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32506) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32506_rabbit-plasma-kallikrein-klkb1-mab-je31-29-298202414333669.jpg)
![Western Blot: Plasma Kallikrein 1B Overexpression Lysate (Adult Normal) [NBL1-12359] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Plasma Kallikrein 1B.](http://images.novusbio.com/fullsize/Plasma-Kallikrein-1B-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12359-img0002.jpg "Western Blot: Plasma Kallikrein 1B Overexpression Lysate (Adult Normal) [NBL1-12359] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Plasma Kallikrein 1B.")
![Immunohistochemistry: Plasma Kallikrein/KLKB1 Antibody (9F6) [NBP3-15209] - IHC image of Plasma Kallikrein/KLKB1 antibody diluted at 1:100 and staining in paraffin-embedded human liver tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/Plasma-Kallikrein-KLKB1-Antibody-9F6-Immunohistochemistry-NBP3-15209-img0001.jpg "Immunohistochemistry: Plasma Kallikrein/KLKB1 Antibody (9F6) [NBP3-15209] - IHC image of Plasma Kallikrein/KLKB1 antibody diluted at 1:100 and staining in paraffin-embedded human liver tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")