Plasma Kallikrein/KLKB1 Antibody [Alexa Fluor® 647]

• Reactivity: Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 647

Plasma Kallikrein/KLKB1 Antibody [Alexa Fluor® 647] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Kallikrein/KLKB1
  Gly20-Ala638
  Accession # NP_032481
• Specificity: Detects mouse Plasma Kallikrein/KLKB1 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross‑reactivity with recombinant human KLKB1 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Plasma Kallikrein/KLKB1 Antibody [Alexa Fluor® 647]

• EC 3.4.21
• EC 3.4.21.34
• Fletcher factor
• kallikrein B, plasma (Fletcher factor) 1
• kininogenin
• KLK3plasma kallikrein
• KLKB1
• plasma kallikrein heavy chain
• plasma kallikrein light chain
• Plasma Kallikrein
• Plasma Prekallikrein
• PPK

Background

Plasma Kallikrein, a serine protease, is synthesized in the liver and circulates in the plasma by binding to high molecular weight (HMW) kininogen or as a free zymogen. Once activated by its physiological activator, factor XII, it displays endopeptidase activity towards peptide bonds after arginine (preferred) and lysine. It cleaves HMW kininogen, its major physiological substrate, to release the potent vasodilator peptide bradykinin. It is also able to cleave a number of inactive precursor proteins to generate active products, such as plasminogen and prourokinase. Thus, it plays an important role in blood pressure regulation, fibrinolysis, and neutrophil activation (1). Mouse Plasma Kallikrein precursor contains a signal peptide (residues 1 to 19) and a pro form sequence (residues 20 to 638). Upon activation, the pro form is converted to a heavy chain and a light chain, which is linked by disulfide bonds and the latter contains the catalytic domain (2). The mouse Plasma Kallikrein pro form was expressed in the NS0 cells with a foreign signal peptide. After being treated by thermolysin, the purified enzyme is active against a fluorogenic peptide substrate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.