Plasma Kallikrein/KLKB1 Antibody (736811) [Alexa Fluor® 750]

• Reactivity: Hu
• Applications: IHC
• Clone: 736811
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

Plasma Kallikrein/KLKB1 Antibody (736811) [Alexa Fluor® 750] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human KLK-B1
  Gly20-Ala638
  Accession # P03952
• Specificity: Detects human KLK-B1 in direct ELISAs.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Plasma Kallikrein/KLKB1 Antibody (736811) [Alexa Fluor® 750]

• EC 3.4.21
• EC 3.4.21.34
• Fletcher factor
• kallikrein B, plasma (Fletcher factor) 1
• kininogenin
• KLK3plasma kallikrein
• KLKB1
• plasma kallikrein heavy chain
• plasma kallikrein light chain
• Plasma Kallikrein
• Plasma Prekallikrein
• PPK

Background

Human Plasma Kallikrein, a serine protease, is synthesized in the liver and circulates in the plasma by binding to high molecular weight (HMW) kininogen or as a free zymogen. Once activated by its physiological activator, factor XII, it displays endopeptidase activity towards peptide bonds after arginine (preferred) and lysine. It cleaves HMW kininogen, its major physiological substrate, to release the potent vasodilator peptide bradykinin. It is also able to cleave a number of inactive precursor proteins to generate active products, such as plasminogen and prourokinase. Thus, it plays an important role in blood pressure regulation, fibrinolysis, and neutrophil activation (1). Human Plasma Kallikrein precursor contains a signal peptide (residues 1 to 19) and a pro form sequence (residues 20 to 638). Upon activation, the pro form is converted to a heavy chain and a light chain, which is linked by disulfide bonds and the latter contains the catalytic domain (2). The human Plasma Kallikrein pro form was expressed in the NS0 cells with a foreign signal peptide. The purified enzyme is mainly the pro form. When activated by thermolysin, it displays activity against a fluorogenic peptide substrate as described in Activity Assay Protocol. This activity can be inhibited by Human Serpin G1/C1 Inhibitor (Catalog # 2488-PI).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.