Plakophilin-3 Antibody (23E3/4)

Images

 
Immunohistochemistry: Plakophilin-3 Antibody (23E3/4) [NBP1-97674] - IHC localization of plakophilin-3 in hair root sheath of dog skin.
Immunohistochemistry: Plakophilin-3 Antibody (23E3/4) [NBP1-97674] - IHC localization of plakophilin-3 in hair root sheath of dog skin.

Product Details

Summary
Reactivity Hu, Mu, CaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
23E3/4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Plakophilin-3 Antibody (23E3/4) Summary

Immunogen
Clone 23E3/4 is a mouse monoclonal IgG2b antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with a synthetic peptide corresponding to amino acid residues 570-589 of human plakophilin-3 coupled to keyhole limpet hemocyanin via an additional cysteine residue at the N-terminus (CFTPQSRRLRELPLAADALTF).
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PKP3
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:25-1:200
  • Immunohistochemistry-Paraffin 1:25-1:200
  • Immunoprecipitation 1:10-1:500
Application Notes
Clone 23E3/4 is suitable for Immunocytochemistry (methanol- and paraformaldehyde-fixed cells), Immunohistochemistry on frozen and paraffin embedded tissues, Immunoprecipitation and Western blot detection. For optimal results, fixed cells and frozen tissues should be treated with 0.2% Triton-X 100 for, respectively, 5 min and 15 min prior to incubation with primary antibody. Optimal antibody dilution should be determined by titration; recommended range is 1:25 - 1:200 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
Control
Plakophilin-3 Knockout HeLa Cell Lysate

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Plakophilin-3 Antibody (23E3/4)

  • plakophilin 3
  • plakophilin-3

Background

Plakophilin-3 is an Armadillo-like protein present in nuclei and desmosomes of epithelial cells. The expression pattern of this protein seems to be largely restricted to epithelial cell types. Plakophilin-3 can be detected along cell borders in a punctuate staining pattern typical for desmosomal proteins. In addition to the desmosomal immunolocalisation, immunostaining was observed as bright nuclear speckles. Thus, like plakophilin-1 and-2, plakophilin-3 displays a dual intracellular localisation in the desmosomal plaque and in the cell nucleus, and therefore is probably involved in signal transduction pathways between the plasma membrane and the nucleus. The Human protein has a predicted molecular mass of 87 kD.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Plakophilin-3 Antibody (NBP1-97674) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-97674AF350
Alexa Fluor 405 NBP1-97674AF405
Alexa Fluor 488 NBP1-97674AF488
Alexa Fluor 532 NBP1-97674AF532
Alexa Fluor 594 NBP1-97674AF594
Alexa Fluor 647 NBP1-97674AF647
Alexa Fluor 700 NBP1-97674AF700
Alexa Fluor 750 NBP1-97674AF750
DyLight 350 NBP1-97674UV
DyLight 405 NBP1-97674V
DyLight 488 NBP1-97674G
DyLight 550 NBP1-97674R
DyLight 594 NBP1-97674DL594
DyLight 650 NBP1-97674C
DyLight 680 NBP1-97674FR
DyLight 755 NBP1-97674IR
Janelia Fluor 549 NBP1-97674JF549
Janelia Fluor 646 NBP1-97674JF646

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Bioinformatics

Gene Symbol PKP3