Plakophilin-3 Antibody (23E3/4) - BSA Free

Immunohistochemistry: Plakophilin-3 Antibody (23E3/4) [NBP1-97674] - IHC localization of plakophilin-3 in hair root sheath of dog skin.

Immunohistochemistry: Plakophilin-3 Antibody (23E3/4) [NBP1-97674] - IHC localization of plakophilin-3 in hair root sheath of dog skin.

• Reactivity: Hu, Mu, Ca
• Applications: WB, ICC/IF, IHC, IP
• Clone: 23E3/4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Plakophilin-3 Antibody (23E3/4) - BSA Free Summary

• Description: The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
• Immunogen: This antibody is a mouse monoclonal IgG2b antibody derived by fusion of Mouse myeloma cells with spleen cells from a Mouse immunized with a synthetic peptide corresponding to amino acid residues 570-589 of Human plakophilin-3 coupled to keyhole limpet hemocyanin via an additional cysteine residue at the N-terminus (CFTPQSRRLRELPLAADALTF).
• Specificity: This antibody reacts with an epitope located between residues 570-589 in Human plakophilin-3.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PKP3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:25-1:200
  • Immunohistochemistry-Paraffin 1:25-1:200
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:1000
• Application Notes: This antibody is suitable for immunocytochemistry (methanol- and paraformaldehyde-fixed cells), immunohistochemistry on frozen and paraffin-embedded tissues, immunoprecipitation and Western blot detection. For optimal results, fixed cells and frozen tissues should be treated with 0.2% Triton-X 100 for, respectively, 5 min and 15 min prior to incubation with primary antibody. Optimal antibody dilution should be determined by titration; recommended range is 1:25 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications..

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Plakophilin-3 Antibody (23E3/4) - BSA Free

• plakophilin 3
• plakophilin-3

Background

Plakophilin-3 is an Armadillo-like protein present in nuclei and desmosomes of epithelial cells. The expression pattern of this protein seems to be largely restricted to epithelial cell types. Plakophilin-3 can be detected along cell borders in a punctuate staining pattern typical for desmosomal proteins. In addition to the desmosomal immunolocalisation, immunostaining was observed as bright nuclear speckles. Thus, like plakophilin-1 and-2, plakophilin-3 displays a dual intracellular localisation in the desmosomal plaque and in the cell nucleus, and therefore is probably involved in signal transduction pathways between the plasma membrane and the nucleus. The Human protein has a predicted molecular mass of 87 kD.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PKP3