Plakophilin-3 Antibody (23E3/4) [Alexa Fluor® 488]

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Product Details

Summary
Reactivity Hu, Mu, CaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
23E3/4
Clonality
Monoclonal
Host
Mouse

Plakophilin-3 Antibody (23E3/4) [Alexa Fluor® 488] Summary

Immunogen
Clone 23E3/4 is a mouse monoclonal IgG2b antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with a synthetic peptide corresponding to amino acid residues 570-589 of human plakophilin-3 coupled to keyhole limpet hemocyanin via an additional cysteine residue at the N-terminus (CFTPQSRRLRELPLAADALTF).
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PKP3
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
Alexa Fluor 488
Format
Alexa Fluor 488 (A=488, E=515-545)
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Application Notes
Clone 23E3/4 is suitable for Immunocytochemistry (methanol- and paraformaldehyde-fixed cells), Immunohistochemistry on frozen and paraffin embedded tissues, Immunoprecipitation and Western blot detection. For optimal results, fixed cells and frozen tissues should be treated with 0.2% Triton-X 100 for, respectively, 5 min and 15 min prior to incubation with primary antibody. Optimal antibody dilution should be determined by titration; recommended range is 1:25 - 1:200 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.

Reactivity Notes

Human, Mouse and Canine. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Alternate Names for Plakophilin-3 Antibody (23E3/4) [Alexa Fluor® 488]

  • plakophilin 3
  • plakophilin-3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Plakophilin-3 Antibody (NBP1-97674AF488) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 647 Labeled NBP1-97674AF647
Alexa Fluor 700 Labeled NBP1-97674AF700

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol PKP3

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