Western Blot: PLA2R1 Antibody [NBP1-84449] - Analysis in human kidney tissue.
Orthogonal Strategies: Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449] - Staining in human kidney and pancreas tissues. Corresponding PLA2R1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449] - Immunohistochemical staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449] - Immunohistochemical staining of human kidney (idiopathic membranous nephropathy) shows strong membranous positivity in cells in glomeruli.
Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449] - Staining of human kidney shows weak membranous positivity in cells in glomeruli.
This antibody was developed against Recombinant Protein corresponding to amino acids: EEKTWHEALRSCQADNSALIDITSLAEVEFLVTLLGDENASETWIGLSSNKIPVSFEWSNDSSVIFTNWHTLEPHIFPNRSQLCVSAEQSEGHWKVKNCEERLFYICKKAGHVLSDAESGCQEGWERHGGFCYKID
Specificity of human PLA2R1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Immunogen affinity purified
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FAQs for PLA2R1 Antibody (NBP1-84449). (Showing 1 - 1 of 1 FAQs).
How many test can be performed in 0.1 ml of PLA2R1 Antibody (NBP1-84449). Also kindly suggest the secondary Antibody for the same.
The number of tests using NBP1-84449 will depend upon the sample type (differential tissues expression) and the total volume of antibody used in each slide. This product is supplied in 100ul size and the concentration of the antibody is 0.4ug/ul and the working dilution we recommend for IHC is 1:500 to 1:1000, based on this information you should be able to estimate how many experiments can be done with one vial. As far as secondary antibody is concerned, any anti rabbit IgG secondary that is conjugated should technical work, the type of conjugation does not matter as much, HRP is most often used in labs while the biotin-conjugated antibody is used to amplify the signal which results in greater sensitivity than that achieved with an enzyme or fluorescence conjugated secondary antibody alone. Fluorescent labeled antibodies are commonly used for double or multiple staining methods. Here is the list of our secondary antibodies. I can recommend NB730-H as it has received very good reviews from our customers and it has been cited in 23 publications as well. I hope this answers your question. Please do not hesitate to contact us if you have any further questions.
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