PKN2 Antibody (509105)

Western blot shows lysates of A431 human epithelial carcinoma cell line, TF-1 human erythroleukemic cell line, C2C12 mouse myoblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, L6 rat myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PKN2 Monoclonal Antibody (Catalog # MAB5686) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PKN2 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 509105
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

PKN2 Antibody (509105) Summary

• Immunogen: E. coli-derived recombinant human PKN2
  Asp18-Asn207
  Accession # Q16513
• Specificity: Detects endogenous human, mouse and rat PKN2 in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PKN2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PKN2 Antibody (509105)

• cardiolipin-activated protein kinase Pak2
• EC 2.7.11
• EC 2.7.11.13
• PAK2
• Pak-2
• PKN2
• PRK2
• PRK2MGC150606
• PRKCL2
• PRKCL2PRO2042
• PRO2042
• Protein kinase C-like 2MGC71074
• protein kinase N2
• Protein-kinase C-related kinase 2
• serine/threonine-protein kinase N2

Background

Serine/Threonine Protein Kinase N2 (PKN2), also known as PRK2, is a cytoplasmic kinase that contains three HR1 repeats (aa 44‑295), one C2 domain (aa 330‑463), and one protein kinase domain (aa 657‑916). PKN2 is a downstream effector of RhoA and promotes the activation of several kinases involved in regulation of the actin cytoskeleton, cell cycle, and apoptosis. PKN2 cleavage during apoptosis generates a C‑terminal fragment that inhibits PDK1 and Akt. PKN2 is also important in the intracellular replication of hepatitis C virus and Yersinia pestis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PKN2 Antibody (MAB5686)(1)

Publication using MAB5686

• ER Murray, S Menezes, JC Henry, JL Williams, L Alba-Caste, P Baskaran, I Quétier, A Desai, JJT Marshall, I Rosewell, M Tatari, V Rajeeve, F Khan, J Wang, P Kotantaki, EJ Tyler, N Singh, CS Reader, EP Carter, K Hodivala-D, RP Grose, HM Kocher, N Gavara, O Pearce, P Cutillas, JF Marshall, AJM Cameron Disruption of pancreatic stellate cell myofibroblast phenotype promotes pancreatic tumor invasion Cell Reports, 2022-01-25;38(4):110227. 2022-01-25 [PMID: 35081338] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: PKN2
• Uniprot:
  • Q16513()