PKM2 Antibody

Images

 
Western Blot: PKM2 Antibody [H00005315-D01P] - Analysis of PKM2 expression in transfected 293T cell line by PKM2 polyclonal antibody.Lane 1: PKM2 transfected lysate(57.90 KDa).Lane 2: Non-transfected lysate.
Western Blot: PKM2 Antibody [H00005315-D01P] - Western Blot analysis of HeLa lysates using H00005315-D01P. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).
Proximity Ligation Assay: PKM2 Antibody [H00005315-D01P] - Analysis of protein-protein interactions between PKM2 and ARAF. HeLa cells were stained with anti-PKM2 rabbit purified polyclonal 1:1200 and anti-ARAF mouse ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PKM2 Antibody Summary

Immunogen
PKM2 (NP_002645.3, 1 a.a. - 531 a.a.) full-length human protein. MSKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP
Specificity
PKM2 - pyruvate kinase, muscle,
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PKM
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Proximity Ligation Assay
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.
Reviewed Applications
Read 1 Review rated 4
using
H00005315-D01P in the following applications:

Publications
Read Publications using H00005315-D01P.

Reactivity Notes

Rat reactivity reported in customer review.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PKM2 Antibody

  • CTHBP
  • Cytosolic thyroid hormone-binding protein
  • MGC3932
  • OIP3
  • OIP-3
  • OIP3EC 2.7.1.40
  • Opa-interacting protein 3
  • p58
  • PK, muscle type
  • PK2
  • PK3
  • PK3PKM
  • PKM2
  • Pyruvate kinase 2/3
  • pyruvate kinase isozymes M1/M2
  • Pyruvate kinase muscle isozyme
  • pyruvate kinase, muscle
  • TCB
  • THBP1
  • THBP1p58
  • Thyroid hormone-binding protein 1
  • thyroid hormone-binding protein, cytosolic
  • Tumor M2-PK

Background

The protein encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF8519
Species: Hu, Mu, Rt
Applications: WB
NBP2-43853
Species: Hu, Mu, Rt, Bv
Applications: WB, ELISA, ICC/IF, IHC-Fr, IHC-P
NB100-2901
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-P
NBP1-84168
Species: Hu
Applications: IHC, IHC-P
NBP2-48704
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-77266
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IF
NBP2-29931
Species: Hu
Applications: WB, Flow, IHC-P
NBP2-03381
Species: Hu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
MAB2014
Species: Hu
Applications: Flow, CyTOF-reported
NBP2-57614
Species: Hu
Applications: ICC/IF
NB100-74398
Species: Hu, Mu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-33137
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
MAB1058
Species: Hu
Applications: Flow, Block, CyTOF-ready
NB100-128
Species: Hu, Mu, Rt
Applications: ChIP, GS, ICC/IF, IHC, IHC-P
NBP1-92290
Species: Hu
Applications: IHC, IHC-P
NBP1-90149
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
MAB4655
Species: Hu
Applications: Flow, CyTOF-ready
NBP1-06274
Species: Hu, Mu, Rt, Ch, Dr, Sh
Applications: WB, Simple Western, Flow, ICC/IF
NBP2-50419
Species: Hu
Applications: Flow, IP, CyTOF-ready
H00005315-D01P
Species: Hu, Rt
Applications: WB, PLA

Publications for PKM2 Antibody (H00005315-D01P)(2)

Review for PKM2 Antibody (H00005315-D01P) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using H00005315-D01P:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot PKM2 H00005315-D01P
Enlarge
reviewed by:
Anonymous
WB Human 01/26/2010
View

Summary

ApplicationWestern Blot
Sample TestedHeLa cell lysates, Sample Amount: 50
SpeciesHuman
Lot08245WULz

Blocking

Blocking DetailsBlocking Buffer: 5% milk, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 1/500, Incubation Dilution Buffer: 5 % milk, Incubation Time: overnight, Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: Donkey anti-rabbit IgG-HRP

Details

Detection NotesDetection Method: ECL Plus, Specific Bands: 58 kDa

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PKM2 Antibody (H00005315-D01P) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional PKM2 Products

Bioinformatics Tool for PKM2 Antibody (H00005315-D01P)

Discover related pathways, diseases and genes to PKM2 Antibody (H00005315-D01P). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PKM2 Antibody (H00005315-D01P)

Discover more about diseases related to PKM2 Antibody (H00005315-D01P).
 

Pathways for PKM2 Antibody (H00005315-D01P)

View related products by pathway.

PTMs for PKM2 Antibody (H00005315-D01P)

Learn more about PTMs related to PKM2 Antibody (H00005315-D01P).
 

Research Areas for PKM2 Antibody (H00005315-D01P)

Find related products by research area.

Blogs on PKM2.

MAPK3/ERK1 - A signal transduction pathway with roles in development and disease
Mitogen-activated protein kinases (MAPKs) are important signaling proteins needed to transmit and relay extracellular stimuli and to illicit intracellular responses (1). The MAPK family of proteins are serine/threonine kinases that are able to phos...  Read full blog post.

5 Stars for the PKM2 Antibody
Novus' Pyruvate Kinase M2 antibody (cat # NBP1-48308) has received some glowing product reviews and customer feedback lately. One satisfied customer wrote, "The best thing about this antibody is that it works well for immunofluorescence staining of fr...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
01/26/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol PKM
Entrez
Uniprot