PKM2 Antibody

Western Blot: PKM2 Antibody [H00005315-D01P] - Analysis of PKM2 expression in transfected 293T cell line by PKM2 polyclonal antibody.Lane 1: PKM2 transfected lysate(57.90 KDa).Lane 2: Non-transfected lysate.

Western Blot: PKM2 Antibody [H00005315-D01P] - Western Blot analysis of HeLa lysates using H00005315-D01P. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).

Proximity Ligation Assay: PKM2 Antibody [H00005315-D01P] - Analysis of protein-protein interactions between PKM2 and ARAF. HeLa cells were stained with anti-PKM2 rabbit purified polyclonal 1:1200 and anti-ARAF mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Product Discontinued

PKM2 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: PKM2 (NP_002645.3, 1 a.a. - 531 a.a.) full-length human protein. MSKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP
• Specificity: PKM2 - pyruvate kinase, muscle,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PKM
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Proximity Ligation Assay
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Reactivity Notes

Rat reactivity reported in customer review.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PKM2 Antibody

• CTHBP
• Cytosolic thyroid hormone-binding protein
• MGC3932
• OIP3
• OIP-3
• OIP3EC 2.7.1.40
• Opa-interacting protein 3
• p58
• PK, muscle type
• PK2
• PK3
• PK3PKM
• PKM2
• Pyruvate kinase 2/3
• pyruvate kinase isozymes M1/M2
• Pyruvate kinase muscle isozyme
• pyruvate kinase, muscle
• TCB
• THBP1
• THBP1p58
• Thyroid hormone-binding protein 1
• thyroid hormone-binding protein, cytosolic
• Tumor M2-PK

Background

The protein encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PKM2 Antibody (H00005315-D01P)(2)

Publications using H00005315-D01P

• Luo W, Hu H, Chang R et al. Pyruvate Kinase M2 is a PHD3-Stimulated Coactivator for Hypoxia-Inducible Factor 1. Cell;145(5):732-44. 2011-05-27 [PMID: 21620138]
• Boutz DR, Collins PJ, Suresh U et al. Two-tiered Approach Identifies a Network of Cancer and Liver Disease-related Genes Regulated by miR-122. J Biol Chem 2011-03-14 [PMID: 21402708]

Review for PKM2 Antibody (H00005315-D01P) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 01/26/2010

Summary

• Application: Western Blot
• Sample Tested: HeLa cell lysates, Sample Amount: 50
• Species: Human
• Lot: 08245WULz

Bioinformatics

• Gene Symbol: PKM
• Entrez:
  • 5315(Human)
• Uniprot:
  • NP_002645.3(Human)