PKM2 Antibody (937801) [Janelia Fluor® 525]

• Reactivity: Hu, Rt
• Applications: WB, IHC, ICC/IF, KO
• Clone: 937801
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 525

PKM2 Antibody (937801) [Janelia Fluor® 525] Summary

• Immunogen: E. coli-derived recombinant human PKM2
  Ser2-Pro531
  Accession # P14618-1
• Specificity: Detects human PKM2 in direct ELISAs. Detects human and rat PKM1/2 in Western blot.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PKM
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Knockout Validated
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for PKM2 Antibody (937801) [Janelia Fluor® 525]

• CTHBP
• Cytosolic thyroid hormone-binding protein
• MGC3932
• OIP3
• OIP-3
• OIP3EC 2.7.1.40
• Opa-interacting protein 3
• p58
• PK, muscle type
• PK2
• PK3
• PK3PKM
• PKM2
• Pyruvate kinase 2/3
• pyruvate kinase isozymes M1/M2
• Pyruvate kinase muscle isozyme
• pyruvate kinase, muscle
• TCB
• THBP1
• THBP1p58
• Thyroid hormone-binding protein 1
• thyroid hormone-binding protein, cytosolic
• Tumor M2-PK

Background

PKM2 is encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PKM