Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
Preservative
No Preservative
Purity
Immunogen affinity purified
Notes
Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.
Alternate Names for PKD2L1 Antibody
PCL
PKD2Lpolycystin-L
PKDLpolycystin-L1
polycystic kidney disease 2-like 1 protein
polycystic kidney disease 2-like 1
Polycystin-2 homolog
polycystin-2L1
Polycystin-L
Polycystin-L1
transient receptor potential cation channel, subfamily P, member 3
TRPP3
Background
This gene encodes a member of the polycystin protein family. The encoded protein contains multiple transmembrane domains, and cytoplasmic N- and C-termini. The protein may be an integral membrane protein involved in cell-cell/matrix interactions. This protein functions as a calcium-regulated nonselective cation channel. Alternative splice variants have been described but their full length sequences have not been determined. [provided by RefSeq]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.
![ELISA: PKD2L1 Antibody [44260002]](http://images.novusbio.com/fullsize/44260002-T01569A01.jpg "ELISA: PKD2L1 Antibody [44260002]")
![Western Blot: ATP Citrate Lyase Antibody [NBP1-90266] - Analysis using Anti-ACLY antibody NBP1-90266 (A) shows similar pattern to independent antibody NBP1-90268 (B).](http://images.novusbio.com/images2/ATP-Citrate-Lyase-Antibody-Western-Blot-NBP1-90266-img0010.jpg)
![Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody [NBP1-90266] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/images2/ATP-Citrate-Lyase-Antibody-Immunohistochemistry-Paraffin-NBP1-90266-img0019.jpg)
![Western Blot: ATP Citrate Lyase Antibody [NBP1-90266] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ACLY antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/images2/ATP-Citrate-Lyase-Antibody-Western-Blot-NBP1-90266-img0007.jpg)
![Western Blot: Polycystin 2 Antibody [NBP1-30117] - Whole cell lysate (15 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Polycystin-2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/images2/Polycystin-2-Antibody-Western-Blot-NBP1-30117-img0007.jpg)
![Western Blot: Polycystin 2 Antibody [NBP1-30117] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Polycystin-2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/images2/Polycystin-2-Antibody-Western-Blot-NBP1-30117-img0006.jpg)
![Immunoprecipitation: Polycystin 2 Antibody [NBP1-30117] - Detection of human Polycystin-2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Polycystin-2 antibody NBP1-30117 used for IP at 3 ug per reaction. Polycystin-2 was also immunoprecipitated by another rabbit anti-Polycystin-2 antibody. For blotting immunoprecipitated Polycystin-2, NBP1-30117 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/images2/Polycystin-2-Antibody-Immunoprecipitation-NBP1-30117-img0005.jpg)
![Western Blot: DNA Polymerase gamma Antibody [NBP1-33633] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA polymerase gamma antibody diluted by 1:1000.](http://images.novusbio.com/images2/DNA-Polymerase-gamma-Antibody-Western-Blot-NBP1-33633-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: DNA Polymerase gamma Antibody [NBP1-33633] - analysis of methanol-fixed HeLa.](http://images.novusbio.com/images2/DNA-Polymerase-gamma-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33633-img0008.jpg)
![Immunohistochemistry-Paraffin: DNA Polymerase gamma Antibody [NBP1-33633] - Paraffin-embedded Skeletal Muscle, using antibody 10 ug/ml.](http://images.novusbio.com/images2/DNA Polymerase gamma Antibody-Immunohistochemistry-Paraffin-NBP1-33633-img0005.jpg)
![Western Blot: Fumarase Antibody [NBP1-31336] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/images2/Fumarase-Antibody-Western-Blot-NBP1-31336-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Fumarase Antibody [NBP1-31336] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Fumarate hydratase protein stained by Fumarate hydratase antibody diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/images2/Fumarase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31336-img0014.jpg)
![Immunohistochemistry-Paraffin: Fumarase Antibody [NBP1-31336] - Analysis of paraffin-embedded NCIN87 xenograft, using fumarate hydratase antibody at 1:500 dilution.](http://images.novusbio.com/images2/Fumarase-Antibody-Immunohistochemistry-Paraffin-NBP1-31336-img0009.jpg)
![Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. Image courtesy of anonymous customer product review.](http://images.novusbio.com/images2/Albumin-Antibody-Western-Blot-NB600-41532-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Albumin Antibody [NB600-41532] - NLRP12 downregulates multiple JNK activating pathways in hepatocytes. For checking the purity of primary hepatocytes, hepatocytes isolated from healthy WT and Nlrp12-/- mouse livers were immunostained with antibody for hepatocyte-specific marker Albumin and counterstained with DAPI. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/40396), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Albumin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-41532-img0003.jpg)
![Immunohistochemistry-Paraffin: Polycystin-1/PKD1 Antibody [NBP1-52552] - Analysis of anti-PKD1 antibody with human kidney at concentration 3.75 ug/ml.](http://images.novusbio.com/images2/Polycystin-1-PKD1-Antibody-Immunohistochemistry-Paraffin-NBP1-52552-img0002.jpg)
![Immunohistochemistry-Paraffin: Polycystin-1/PKD1 Antibody [NBP1-52552] - Analysis of anti-PKD1 antibody with human spleen at concentration 3.75 ug/ml.](http://images.novusbio.com/images2/Polycystin-1-PKD1-Antibody-Immunohistochemistry-Paraffin-NBP1-52552-img0001.jpg)
![Western Blot: Afamin Antibody [NBP2-15304] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7. 5% SDS PAGE; antibody diluted at 1:5000.](http://images.novusbio.com/images2/AFM Antibody-Western Blot-NBP2-15304-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Afamin Antibody [NBP2-15304] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/images2/AFM Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15304-img0001.jpg)
![Immunohistochemistry-Paraffin: Afamin Antibody [NBP2-15304] - Paraffin-embedded human colon. AFM antibody diluted at 1:500.](http://images.novusbio.com/images2/Afamin-Antibody-Immunohistochemistry-Paraffin-NBP2-15304-img0005.jpg)
![Western Blot: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Non-transfected (Negative) and transfected (Positive) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/images2/CS-Citrate-Synthase-Antibody-(1761)-Western-Blot-NBP2-43648-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: CS Citrate Synthase Antibody (1761) [NBP2-43648] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Citrate synthetase protein stained by Citrate synthetase antibody [1761] diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining.](http://images.novusbio.com/images2/CS-Citrate-Synthase-Antibody-(1761)-Immunocytochemistry-Immunofluorescence-NBP2-43648-img0012.jpg)
![Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Analysis of mouse kidney. Citrate synthetase antibody [1761] dilution: 1:200.](http://images.novusbio.com/images2/CS-Citrate-Synthase-Antibody-(1761)-Immunohistochemistry-Paraffin-NBP2-43648-img0004.jpg)
![Immunohistochemistry-Paraffin: Glycophorin C Antibody [NBP1-84597] - Analysis in human bone marrow and pancreas tissues using NBP1-84597 antibody. Corresponding GYPC RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Glycophorin-C-Antibody-Immunohistochemistry-Paraffin-NBP1-84597-img0008.jpg)
![Western Blot: Glycophorin C Antibody [NBP1-84597] - Analysis in control (vector only transfected HEK293T lysate) and GYPC over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/Glycophorin-C-Antibody-Western-Blot-NBP1-84597-img0007.jpg)
![Immunohistochemistry-Paraffin: Glycophorin C Antibody [NBP1-84597] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/images2/Glycophorin-C-Antibody-Immunohistochemistry-Paraffin-NBP1-84597-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Stabilin-2 Antibody [NBP1-84197] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/images2/Stabilin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84197-img0006.jpg)
![Immunohistochemistry-Paraffin: Stabilin-2 Antibody [NBP1-84197] - Staining of human testis shows moderate cytoplasmic positivity in endothelial cells.](http://images.novusbio.com/images2/Stabilin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-84197-img0014.jpg)
![Immunohistochemistry-Paraffin: Stabilin-2 Antibody [NBP1-84197] - Analysis in human spleen and cerebral cortex tissues. Corresponding STAB2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Stabilin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-84197-img0010.jpg)
![Western Blot: Fibronectin Antibody [NBP1-91258] - VSOP observed in perivascular-restricted spinal cord lesions with intact BBB. Immunostaining for laminin (brown) shows vascular endothelium and glia limitans of a perivascular lesion, along with infiltrating cells and VSOP (blue). Image collected and cropped by CiteAb from the following publication (http://asn.sagepub.com/lookup/doi/10.1042/AN20120081), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Fibronectin-Antibody-Western-Blot-NBP1-91258-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: Fibronectin Antibody [NBP1-91258] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Fibronectin at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/Fibronectin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91258-img0012.jpg)
![Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Analysis in a 6 month old Alport mouse kidney. Image from a verified customer review.](http://images.novusbio.com/images2/Fibronectin-Antibody-Immunohistochemistry-Frozen-NBP1-91258-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: PKC mu Antibody [NBP1-87789] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.](http://images.novusbio.com/images2/PKC mu Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87789-img0007.jpg)
![Immunohistochemistry-Paraffin: PKC mu Antibody [NBP1-87789] - Staining of human parathyroid gland shows cytoplasmic positivity in glandular cells.](http://images.novusbio.com/images2/PKC-mu-Antibody-Immunohistochemistry-Paraffin-NBP1-87789-img0006.jpg)
![Western Blot: Musculin Antibody [NBP2-56244] - Western blot analysis in human cell line RT-4.](http://images.novusbio.com/images2/Musculin Antibody-Western Blot-NBP2-56244-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Musculin Antibody [NBP2-56244] - Staining of human cell line SK-MEL-30 shows localization to nucleoplasm.](http://images.novusbio.com/images2/Musculin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56244-img0001.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")