PKC zeta Antibody (2D1) - Azide and BSA Free

Western Blot: PKC zeta Antibody (2D1) [H00005590-M01] - Analysis of PRKCZ expression in transfected 293T cell line by PRKCZ monoclonal antibody (M01), clone 2D1.Lane 1: PRKCZ transfected lysate(67.7 KDa).Lane 2: Non-transfected lysate.

Western Blot: PKC zeta Antibody (2D1) [H00005590-M01] - Analysis of PRKCZ over-expressed 293 cell line, cotransfected with PRKCZ Validated Chimera RNAi ( Cat # H00005590-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PRKCZ monoclonal antibody (M01) clone 2D1 (Cat # H00005590-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Sandwich ELISA: PKC zeta Antibody (2D1) [H00005590-M01] - Detection limit for recombinant GST tagged PRKCZ is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, KD
• Clone: 2D1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PKC zeta Antibody (2D1) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PRKCZ (AAH08058, 165 a.a. ~ 255 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KLLVHKRCHGLVPLTCRKHMDSVMPSQEPPVDDKNEDADLPSEETDGIAYISSSRKHDSIKDDSEDLKPVIDGMDGIKISQGLGLQDFDLI
• Localization: Cytoplasmic and endosome
• Specificity: PRKCZ - protein kinase C, zeta
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRKCZ
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PKC zeta Antibody (2D1) - Azide and BSA Free

• EC 2.7.11
• EC 2.7.11.13
• nPKC-zeta
• PKC zeta
• PKC2
• PKC2PKC-ZETA
• PRKCZ
• protein kinase C zeta type
• protein kinase C, zeta

Background

Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKCZ
• Entrez:
  • 5590(Human)
• OMIM:
  • 176982(Human)
• Uniprot:
  • AAH08058(Human)