Recombinant Human PKC delta Protein

Product Discontinued

Recombinant Human PKC delta Protein Summary

• Description: Recombinant protein for Human PKC delta
  
  

  Source:Baculovirus (Sf9 insect cells)
  Amino Acid Sequence:(NM_006254)

• Source: Sf 9 (baculovirus)
• Protein/Peptide Type: Recombinant Protein
• Gene: PRKCD
• Purity: >80% SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Specific activity: 302 nmol phosphate incorporated into CREBtide substrate per minute per mg protein at 30C for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay).
• Theoretical MW: 104 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
• Concentration: 0.1 mg/ml
• Purity: >80% SDS-PAGE

Alternate Names for Recombinant Human PKC delta Protein

• EC 2.7.11
• EC 2.7.11.13
• MAY1
• MGC49908
• nPKC-delta
• PKC delta
• PKCD
• PKCy
• PRKCD
• protein kinase C delta type
• protein kinase C delta VIII
• protein kinase C, delta

Background

Protein kinase C delta (PKC delta) is a member of the protein kinase C (PKC) family of serine-threonine kinases. It is a 79 kd protein kinase that shows strict dependence on the presence of phospholipids, but shows no activation by Ca2+ (1). Phosphatidylinositol is the most potent activator of PKC delta. Apparent kinetic constants for synthetic oligopeptides (MBP4-14, EGFR peptide and epsilon-peptide) are quite different between PKC delta and other PKCs. Northern blot analysis indicated that PKC delta is widely distributed in almost all the tissues and is a major isoform of PKC expressed in hemopoietic cells (2). PKC delta is involved in fundamental cellular functions regulated by diacylglycerols and mimicked by phorbol esters. PKC delta is partially associated with the insoluble fraction in cells even in the absence of phorbol 12-myristate 13-acetate (PMA). Upon PMA stimulation, both it translocate to the insoluble fraction of cell homogenates (3). Overexpression of PKC-delta induces significant changes in morphology and causes the cells to grow more slowly and to a decreased cell density in confluent cultures. These changes are accentuated by treatment with PMA. None of the PKC-delta overexpressers grow in soft agar with or without PMA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PRKCD