Recombinant Human PKC beta Protein

Product Discontinued

Recombinant Human PKC beta Protein Summary

• Description: Recombinant protein for Human PKC beta
  
  Source:Baculovirus (Sf9 insect cells)
  Amino Acid Sequence:(NM_002738)
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab60841)
• Preparation Method: Determined to be >79% pure by SDS-PAGE
• Source: Sf 9 (baculovirus)
• Protein/Peptide Type: Recombinant Protein
• Gene: PRKCB
• Purity: >79%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Specific activity: 339 nmol phosphate incorporated into CREBtide per minute per mg protein at 30C for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay).
• Theoretical MW: 105 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
• Concentration: 0.1 mg/ml
• Purity: >79%, by SDS-PAGE

Alternate Names for Recombinant Human PKC beta Protein

• EC 2.7.11
• EC 2.7.11.13
• MGC41878
• PKC-B
• PKC-beta
• PKCBPRKCB2
• PRKCB1protein kinase C beta 1
• protein kinase C beta type
• protein kinase C, beta 1 polypeptide
• protein kinase C, beta 1
• protein kinase C, beta

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Greenham determined the genomic structure of the PRKCBeta gene, which consists of 18 exons spanning 375 kb (1). PRKCBeta has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Leitges found that the 2 isoforms, PRKCBeta 1 and PRKCBeta 2, play an important role in B-cell activation and may be functionally linked to Bruton tyrosine kinase in antigen receptor-mediated signal transduction (2). Su proposed that PRKCBeta inhibitors and inhibitors of other PRKC isoforms may be effective in treating disorders characterized by dysregulated NFKB survival signaling (3). Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PRKCB