PKC alpha Antibody (MC5)

Product Discontinued

PKC alpha Antibody (MC5) Summary

• Immunogen: Purified bovine brain protein kinase C alpha
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRKCA
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/ml
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Frozen
  • Immunoprecipitation 12.5 ug/ml
  • Western Blot 1:1000
• Application Notes: Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 26011242)
• Theoretical MW: 77 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Mouse Brain Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Cross-reacts with Human, Rat, Mouse, Cow, Dog, Fish, Guinea Pig, Monkey, Pig, Rabbit and Sheep. Not yet tested in other species. Zebrafish reactivity reported in scientific literature (PMID: 26246494)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for PKC alpha Antibody (MC5)

• AAG6
• aging-associated gene 6
• EC 2.7.11
• EC 2.7.11.13
• MGC129901
• PKC alpha
• PKCA
• PKC-A
• PKC-alpha
• PKCAMGC129900
• PRKACA
• PRKCA
• protein kinase C alpha type
• protein kinase C, alpha

Background

Protein Kinase C (PKC) is a large superfamily of serine/threonine kinases that mediate essential cellular signals required for activation, proliferation, differentiation and survival. There are at least ten PKC isotypes that are closely related in structure but that have distinct patterns of tissue distribution and function. The PKC isotypes can be subdivided into three classes based on primary structure and biochemical properties. These are: classical or conventional PKC isotypes (cPKC), novel PKC isotypes (nPKC) and atypical PKC isotypes (aPKC). All PKC isotypes share a characteristic equence motif C1 in addition to a serine/threonine-protein kinase domain. The cPKC isotypes include PKC

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PKC alpha Antibody (NB200-586)(6)

Publications using NB200-586

• Zang J, Keim J, Kastenhuber E et al. Recoverin depletion accelerates cone photoresponse recovery. Open Biol. 2015-08-01 [PMID: 26246494] (IF/IHC, Zebrafish)
• Hovhannisyan A, Benkner B, Biesemeier A et al. Effects of the jimpy mutation on mouse retinal structure and function. J. Comp. Neurol. 2015-05-23 [PMID: 26011242] (WB, IHC-Fr, Mouse)
• Schubert T, Gleiser C, Heiduschka P et al. Deletion of myosin VI causes slow retinal optic neuropathy and age-related macular degeneration (AMD)-relevant retinal phenotype. Cell. Mol. Life Sci. 2015-05-06 [PMID: 25939269] (IF/IHC, Mouse)
• Niklaus S, Glasauer S, Kovermann P et al. Glutamate Transporters are Involved in Direct Inhibitory Synaptic Transmission in the Vertebrate Retina bioRxiv 2023-05-01 (IHC-Fr, Zebrafish)
• Huang YY, Haug MF, Gesemann M, Neuhauss SC. Novel expression patterns of metabotropic glutamate receptor 6 in the zebrafish nervous system. PLoS One;7(4). 2012-01-01 [PMID: 22523578] (ICC/IF, Xenografts)
• Zhang, C-L, Zou, Y, Yu, R T, Gage, F H, Evans, R M. Nuclear receptor TLX prevents retinal dystrophy recruits the corepressor atrophin1. 20, 1308-1320. 2006-01-01 [PMID: 16702404]

FAQs for PKC alpha Antibody (NB200-586). (Showing 1 - 1 of 1 FAQs).

1. We are looking into protein kinase C-alpha antibodies for immunohistochemistry on formalin fixed, paraffin embedded tissue. I see that you have many antibodies, including several monoclonals. I wanted to confirm with you that the following antibodies are specific for C-alpha. NB600-201=MC5 (an older paper suggested that this might react with alpha, beta, gamm), NB110-57356=Y124, NB110-57353=Y143. Also, if you have an comparator data or feedback regarding PKC-alpha antibodies for IHC on FFPE, that would be very helpful. We would like to pick 1 antibody that is easy to titer and robust, so that we can move forward with our studies.
   • We have never heard any feedback that these cross-react with other PKC proteins. If you have seen data that MC5 may cross-react, then I would consider avoiding this antibody if you are concerned. The immunogens for NB110-57356 and NB110-57353 come from the N- and C-terminals, which is where the PKC proteins vary the most, so these are not expected to cross-react and we have had no feedback or testing data that suggests that they do. I would recommend NB110-57356, since it has been published with. You may want to review the published data to determine if it seems this antibody will suit your needs.

Bioinformatics

• Gene Symbol: PRKCA
• Entrez:
  • 5578(Human)
  • 18750(Mouse)
  • 25636(Rat)