PKA R2 [p Ser96] Antibody

Western Blot: PKA R2 [p Ser96] Antibody [NBP3-12928] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKA R2 (phospho Ser96) antibody (NBP3-12928) diluted at 1:3000. The signal was developed with Trident ECL plus-Enhanced.

Untreated (-) and treated (+) C6 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKA R2 (phospho Ser96) antibody (NBP3-12928) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

• Reactivity: Hu, Pm
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PKA R2 [p Ser96] Antibody Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Carrier-protein conjugated synthetic peptide surrounding phospho Ser96 of human PKA R2. The exact sequence is proprietary.
• Modification: p Ser96
• Predicted Species: Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRKAR2A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for PKA R2 [p Ser96] Antibody

• protein kinase, cAMP-dependent, regulatory, type II, alpha
• RII-alpha subunit

Background

PKA (or cAPK) is a cyclic AMP-dependent protein kinase. When activated by the second messenger cAMP, PKA mediates diverse cellular mechanisms, including proliferation, ion transport, regulation of metabolism, and gene transcription (1,2). PKA is comprised of two dimers of two subunits, R (regulatory) and C (catalytic). Two families of R subunits (RI and RII) have been identified, each possessing distinct cAMP binding properties and resulting in different phosphorylation states (2,3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKAR2A