PKA R2 [p Ser99] Antibody (HL3583)

Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKA R2 antibody [HL3583] (NBP3-48829) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

• Reactivity: Mu
• Applications: WB
• Clone: HL3583
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

PKA R2 [p Ser99] Antibody (HL3583) Summary

• Description: Novus Biologicals Rabbit PKA R2 [p Ser99] Antibody (HL3583) (NBP3-48829) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Carrier-protein conjugated synthetic peptide surrounding phospho Ser99 of human PKA R2. The exact sequence is proprietary.
• Modification: p Ser99
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PRKAR2A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for PKA R2 [p Ser99] Antibody (HL3583)

• protein kinase, cAMP-dependent, regulatory, type II, alpha
• RII-alpha subunit

Background

PKA (or cAPK) is a cyclic AMP-dependent protein kinase. When activated by the second messenger cAMP, PKA mediates diverse cellular mechanisms, including proliferation, ion transport, regulation of metabolism, and gene transcription (1,2). PKA is comprised of two dimers of two subunits, R (regulatory) and C (catalytic). Two families of R subunits (RI and RII) have been identified, each possessing distinct cAMP binding properties and resulting in different phosphorylation states (2,3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKAR2A