PITX1 Antibody

Product Discontinued

PITX1 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 100-150 of Human Pitx1.
• Specificity: Pitx1 (E130) detects endogenous levels of Pitx1 protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PITX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of Pitx1 (E130) pAb in extracts from Jurkat cells.
  Immunohistochemistry (IHC) analyzes of Pitx1 (E130) pAb in paraffin-embedded human lung carcinoma tissue.
• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PITX1 Antibody

• BFTpituitary homeobox 1
• CCF
• Hindlimb-expressed homeobox protein backfoot
• Homeobox protein PITX1
• paired-like homeodomain 1
• pituitary otx-related factor
• POTXpituitary homeo box 1
• PTX1Paired-like homeodomain transcription factor 1hindlimb expressed homeobox protein backfoot

Background

Pitx1 and Pitx2 are highly homologous, bicoid-related transcription factors. Pitx1 is a bicoid-related homeodomain factor that exhibits preferential expression in the hindlimb, as well as expression in the developing anterior pituitary gland and first branchial arch. Deletion of the Pitx1 locus results in decreased distal expression of the hindlimb-specific marker, the T-box factor (Tbx4). Pitx1 may modulate morphogenesis, growth and patterning of a specific hindlimb region, and serves as a component of the variables that influence morphological and growth distinctions in forelimb and hindlimb identity. Pitx2 was initially identified as the gene responsible for human Rieger syndrome, an autosomal dominant condition that causes developmental abnormalities. Pitx2 is a transcription factor that regulates cardiac positioning and pituitary and tooth morphogenesis. Pitx2 also regulates lung symmetry by encoding "leftness" of the lung. Pitx2 is asymmetrically expressed in the left lateral-plate mesoderm, and mutant mice with laterality defects show altered patterns of Pitx2 expression that correlate with changes in the visceral symmetry. The genes which encode Pitx1 and Pitx2 map to human chromosomes 5q31 and 4q25-q26, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PITX1 Antibody (NBP1-19686)(1)

Publication using NBP1-19686

• Yu Z, Chen D, Su Z et al. miR-886-3p upregulation in clear cell renal cell carcinoma regulates cell migration, proliferation and apoptosis by targeting PITX1. Int. J Mol. Med. 2014-11-01 [PMID: 25190136]

Bioinformatics

• Gene Symbol: PITX1
• Entrez:
  • 5307(Human)
  • 18740(Mouse)
  • 113983(Rat)
• Uniprot:
  • P78337(Human)