PITPNB Antibody (OTI6H10) - Azide and BSA Free

Western Blot: PITPNB Antibody (OTI6H10) - Azide and BSA Free [NBP2-73421] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PITPNB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti PITPNB.(1:2000)

Immunohistochemistry: PITPNB Antibody (OTI6H10) - Azide and BSA Free [NBP2-73421] - Staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PITPNB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)

Immunohistochemistry: PITPNB Antibody (OTI6H10) - Azide and BSA Free [NBP2-73421] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti PITPNB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)

Immunohistochemistry: PITPNB Antibody (OTI6H10) - Azide and BSA Free [NBP2-73421] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PITPNB mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)

Immunohistochemistry: PITPNB Antibody (OTI6H10) - Azide and BSA Free [NBP2-73421] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PITPNB mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)

Immunohistochemistry: PITPNB Antibody (OTI6H10) - Azide and BSA Free [NBP2-73421] - Staining of paraffin-embedded Human lymphoma tissue using anti-PITPNB mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI6H10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

PITPNB Antibody (OTI6H10) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PITPNB Antibody (OTI6H10) - Azide and BSA Free (NBP2-46422) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human PITPNB (NP_036531) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PITPNB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for PITPNB Antibody (OTI6H10) - Azide and BSA Free

• phosphatidylinositol transfer protein beta isoform
• phosphatidylinositol transfer protein, beta
• phosphotidylinositol transfer protein, beta
• PI-TP-beta
• PtdIns transfer protein beta
• PtdInsTP beta
• PtdInsTP
• VIB1B

Background

PITPNB, also known as Phosphatidylinositol Transfer Protein Beta, has two isoforms that are each approximately 32 kDa with 271 and 272 amino acids, respectively. PITPNB is localized to the cytoplasm and expressed in a variety of tissues, including the brain. PITPNB is responsible for catalyzing the transfer of phosphatidylcholine and phosphatidylinositol between membranes. Pathways in which PITPNB plays a role include phospholipid metabolism and PI/PC transport between the ER and Golgi membranes. Current research on PITPNB is being conducted in relation to obesity, and PITPNB has been shown to interact with Von Hippel Lindau, Ubiquitin C, Lamin A and LMO4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PITPNB