Pirin Antibody

Images

 
Western Blot: Pirin Antibody [NB300-958] - HEK293 overexpressing PIR and probed with (mock transfection in first lane).
Western Blot: Pirin Antibody [NB300-958] - (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Pirin Antibody Summary

Immunogen
Peptide with sequence GSSKKVTLSVLSREC corresponding to N-Terminus according to NP_003653.1.
Specificity
Variants (NP_003653.1; NP_001018119.1) encode the same protein.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PIR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 10 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Approx. 33 kDa band observed in human heart lysate (calculated MW of 32.1 kDa band according to NP_003653.1). In transfected HEK293 transiently expressing PIR a band of approx. 35 kDa is observed. This band is not observed in the non-transfected HEK293.
Positive Control
Pirin Lysate (NBL1-14439)
Publications
Read Publication using NB300-958.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Pirin Antibody

  • EC 1.13.11.24
  • pirin (iron-binding nuclear protein)
  • pirin
  • Probable quercetin 2,3-dioxygenase PIR
  • Probable quercetinase

Background

This gene encodes a member of the cupin superfamily. The encoded protein is an Fe(II)-containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication. Alternatively spliced transcript variants have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Pirin Antibody (NB300-958)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Pirin Antibody (NB300-958)

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Bioinformatics

Gene Symbol PIR
Entrez
Uniprot