Pirin Antibody

Western Blot: Pirin Antibody [NB300-958] - Staining of Human Heart lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Pirin Antibody [NB300-958] - Staining of paraffin embedded Human Spleen. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: Pirin Antibody [NB300-958] - Staining of paraffin embedded Human Liver. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Pirin Antibody Summary

• Description: Novus Biologicals Goat Pirin Antibody (NB300-958) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-Pirin Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence GSSKKVTLSVLSREC corresponding to N-Terminus according to NP_003653.1.
• Specificity: Variants (NP_003653.1; NP_001018119.1) encode the same protein.
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PIR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.3 - 1.0 ug/mL
• Application Notes: WB: Approx. 33 kDa band observed in human heart lysate (calculated MW of 32.1 kDa band according to NP_003653.1).
• Control:

  Pirin Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Pirin Antibody

• EC 1.13.11.24
• pirin (iron-binding nuclear protein)
• pirin
• Probable quercetin 2,3-dioxygenase PIR
• Probable quercetinase

Background

This gene encodes a member of the cupin superfamily. The encoded protein is an Fe(II)-containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication. Alternatively spliced transcript variants have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pirin Antibody (NB300-958)(1)

Publication using NB300-958

• Wendler WM, Kremmer E, Forster R, Winnacker EL. Identification of pirin, a novel highly conserved nuclear protein. J Biol Chem 1997-03-28 [PMID: 9079676]

Bioinformatics

• Gene Symbol: PIR
• Entrez:
  • 8544(Human)
  • 69656(Mouse)
• Uniprot:
  • NP_003653.(Human)