Pirin Antibody (4D1) - Azide and BSA Free

Western Blot: Pirin Antibody (4D1) [H00008544-M03] - Analysis of PIR expression in transfected 293T cell line by PIR monoclonal antibody (M03), clone 4D1. Lane 1: PIR transfected lysatE (32.113 KDa). Lane 2: Non-transfected lysate.

Western Blot: Pirin Antibody (4D1) [H00008544-M03] - Western blot analysis of PIR over-expressed 293 cell line, cotransfected with PIR Validated Chimera RNAi or non-transfected control. Blot probed with H00008544-M03. GAPDH (36.1 kDa) used as loading control.

• Reactivity: Hu
• Applications: WB, ELISA, KD
• Clone: 4D1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Pirin Antibody (4D1) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PIR (AAH02517, 1 a.a. ~ 290 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGSSKKVTLSVLSREQSEGVGARVRRSIGRPELKNLDPFLLFDEFKGGRPGGFPDHPHRGFETVSYLLEGGSMAHEDFCGHTGKMNPGDLQWMTAGRGILHAEMPCSEEPAHGLQLWVNLRSSEKMVEPQYQELKSEEIPKPSKDGVTVAVISGEALGIKSKVYTRTPTLYLDFKLDPGAKHSQPIPKGWTSFIYTISGDVYIGPDDAQQKIEPHHTAVLGEGDSVQVENKDPKRSHFVLIAGEPLREPVIQHGPFVMNTNEEISQAILDFRNAKNGFERAKTRKSKIGN
• Specificity: PIR (4D1)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PIR
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Western Blot
• Application Notes: Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA and RNAi Validation.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Pirin Antibody (4D1) - Azide and BSA Free

• EC 1.13.11.24
• pirin (iron-binding nuclear protein)
• pirin
• Probable quercetin 2,3-dioxygenase PIR
• Probable quercetinase

Background

This gene encodes a member of the cupin superfamily. The encoded protein is an Fe(II)-containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication. Alternatively spliced transcript variants have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PIR
• Entrez:
  • 8544(Human)
• OMIM:
  • 603329(Human)
• Uniprot:
  • AAH02517(Human)