Recombinant Human PIP5K2B His Protein

SDS-Page: PIP5K2B Protein [NBP2-23327]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human PIP5K2B His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-416 of Human PIP5K2B
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSSNCTS TTAVAVAPLS ASKTKTKKKH FVCQKVKLFR ASEPILSVLM WGVNHTINEL SNVPVPVMLM PDDFKAYSKI KVDNHLFNKE NLPSRFKFKE YCPMVFRNLR ERFGIDDQDY QNSVTRSAPI NSDSQGRCGT RFLTTYDRRF VIKTVSSEDV AEMHNILKKY HQFIVECHGN TLLPQFLGMY RLTVDGVETY MVVTRNVFSH RLTVHRKYDL KGSTVAREAS DKEKAKDLPT FKDNDFLNEG QKLHVGEESK KNFLEKLKRD VEFLAQLKIM DYSLLVGIHD VDRAEQEEME VEERAEDEEC ENDGVGGNLL CSYGTPPDSP GNLLSFPRFF GPGEFDPSVD VYAMKSHESS PKKEVYFMAI IDILTPYDTK KKAAHAAKTV KHGAGAEIST VNPEQYSKRF NEFMSNILT

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PIP4K2B
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 49.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 10% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human PIP5K2B His Protein

• 1-phosphatidylinositol-4-phosphate kinase
• 1-phosphatidylinositol-5-phosphate 4-kinase 2-beta
• Diphosphoinositide kinase 2-beta
• EC 2.7.1
• EC 2.7.1.149
• phosphatidylinositol-4-phosphate 5-kinase, type II, beta
• Phosphatidylinositol-5-phosphate 4-kinase type II beta
• phosphatidylinositol-5-phosphate 4-kinase type-2 beta
• phosphatidylinositol-5-phosphate 4-kinase, type II, beta
• PI(5)P 4-kinase type II beta
• PI5P4KB
• PIP4KII-beta
• PIP5K2B
• PIP5KIIB
• PIP5KIIbeta
• PTDINS(4)P-5-kinase
• PtdIns(5)P-4-kinase isoform 2-beta

Background

PIP4K2B catalyzes the phosphorylation of phosphatidylinositol-4-phosphate on the fifth hydroxyl of the myo-inositol ring to form phosphatidylinositol-4,5-bisphosphate. It is a member of the phosphatidylinositol-4-phosphate 5-kinase family. The amino acid sequence of PIP4K2B does not show similarity to other kinases, but the protein does exhibit kinase activity. Additionally, PIP4K2B interacts with p55 TNF receptor. Recombinant human PIP4K2B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PIP4K2B