PIP Antibody (PIP2 2C11)

Product Discontinued

PIP Antibody (PIP2 2C11) Summary

• Immunogen: Liposomes (prepared in PBS with lipid A, phosphatidylcholine (PC) and cholesterol) containing synthetic di-palmitoyl PtdIns(4,5)P2.
• Specificity: Cross reacts with PtdIns(4,5)P2, Ptd(4)P and Ptd(3,4,5)P3.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: PIP
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: Positive control(s): To test the Ab efficiency use a lipid blot assay as described in References above. It is recommended to use a lipid blot assay or nuclear staining to test this antibody.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein A purified

Alternate Names for PIP Antibody (PIP2 2C11)

• GCDFP15prolactin-inducible protein
• GCDFP-15SABP
• GPIP4gp17
• Gross cystic disease fluid protein 15
• prolactin-induced proteinSecretory actin-binding protein

Background

Phosphatidylinositol 4,5-biphosphate (PIP2) has been implicated in a variety of cellular processes, including synaptic vesicle recycling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PIP Antibody (NB100-2751)(1)

Publication using NB100-2751

• Evans, N J, Walker, J W. (2006) Endothelin-1 Mobilizes Profilin-1-Bound PIP2 in Cardiac Muscle, 231, 882-887. [PMID: 16741017]

Bioinformatics

• Gene Symbol: PIP