PIP Antibody (PIP/9076R) - Azide and BSA Free

Formalin-fixed, paraffin-embedded human breast carcinoma stained with PIP antibody (PIP/9076R) - Azide and BSA Free. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

• Reactivity: Hu
• Applications: IHC
• Clone: PIP/9076R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

PIP Antibody (PIP/9076R) - Azide and BSA Free Summary

• Description: Antibody without azide - store at -20 to -80C.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment (around aa 1-146) of human PIP protein (exact sequence is proprietary)
• Localization: Cytoplasm (Golgi bodies). Secreted.
• Marker: Breast Marker
• Specificity: It recognizes a protein of 15kDa, identified as Gross cystic disease fluid protein 15 (GCDFP-15). This antibody is useful in the identification of metastatic breast carcinoma, or fluid analysis.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PIP
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2ug/ml
• Application Notes: Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for PIP Antibody (PIP/9076R) - Azide and BSA Free

• GCDFP15prolactin-inducible protein
• GCDFP-15SABP
• GPIP4gp17
• Gross cystic disease fluid protein 15
• prolactin-induced proteinSecretory actin-binding protein

Background

The androgen and prolactin responsive prolactin-inducible protein (PIP), also known as Gross cystic disease fluid protein (GCDFP-15) is expressed in benign and malignant human breast tumors and in such normal exocrine organs as sweat, salivary and lacrimal glands. The hormonally responsive PIP gene is expressed in benign and malignant breast tumor tissues and in such normal exocrine organs as sweat, salivary, and lacrimal glands. The cells within the body that produce PIP/GCDFP 15 appear to be restricted primarily to those with apocrine function. Studies have found PIP to be a highly specific and sensitive marker for breast cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PIP