Recombinant Human PIM1 Protein

Product Discontinued

Recombinant Human PIM1 Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 38-290 of Human PIM1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MYQVGPLLGS GGFGSVYSGI RVSDNLPVAI KHVEKDRISD WGELPNGTRV PMEVVLLKKV SSGFSGVIRL LDWFERPDSF VLILERPEPV QDLFDFITER GALQEELARS FFWQVLEAVR HCHNCGVLHR DIKDENILID LNRGELKLID FGSGALLKDT VYTDFDGTRV YSPPEWIRYH RYHGRSAAVW SLGILLYDMV CGDIPFEHDE EIIRGQVFFR QRVSSECQHL IRWCLALRPS DRPTFEEIQN HPWM

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PIM1
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 31.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 10% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human PIM1 Protein

• EC 2.7.11.1
• Oncogene PIM1
• PIM
• pim-1 kinase 44 kDa isoform
• pim-1 oncogene (proviral integration site 1)
• pim-1 oncogene
• PIM1
• proto-oncogene serine/threonine-protein kinase pim-1

Background

PIM1 belongs to the Ser/Thr protein kinase family, and PIM subfamily. This protein is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons. Recombinant human Pim1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PIM1