PIM1 Antibody

Western Blot: PIM1 Antibody [NB100-2313] - Whole cell lysate (50 ug) from K562 cells. Antibody used at 0.3 ug/ml.

Product Discontinued

PIM1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 250 and the C-terminus (residue 313) of human PIM1 oncoprotein using the numbering given in Swiss-Prot entry P11309 (GeneID 5292).
• Predicted Species: Bovine (100%), Feline (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PIM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 4-8 ug/mg lysate
  • Western Blot 1:3000-1:15000
• Control:

  PIM1 Overexpression Lysate

Reactivity Notes

Cat (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PIM1 Antibody

• EC 2.7.11.1
• Oncogene PIM1
• PIM
• pim-1 kinase 44 kDa isoform
• pim-1 oncogene (proviral integration site 1)
• pim-1 oncogene
• PIM1
• proto-oncogene serine/threonine-protein kinase pim-1

Background

The protein encoded by this gene is an epithelial-derived, integral membrane serine protease. This protease forms a complex with the Kunitz-type serine protease inhibitor, HAI-1, and is found to be activated by sphingosine 1-phosphate. This protease has been shown to cleave and activate hepatocyte growth factor/scattering factor, and urokinase plasminogen activator, which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors. The expression of this protease has been associated with breast, colon, prostate, and ovarian tumors, which implicates its role in cancer invasion, and metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PIM1 Antibody (NB100-2313)(2)

Publications using NB100-2313

• Prendergast L, van Vuuren C, Kaczmarczyk A et al. Premitotic assembly of human CENPs -T and -W switches centromeric chromatin to a mitotic state. PLoS Biol 2011-06-01 [PMID: 21695110]
• Shankar DB, Li J, Tapang P et al. ABT-869, a multitargeted receptor tyrosine kinase inhibitor: inhibition of FLT3 phosphorylation and signaling in acute myeloid leukemia. Blood 2007-04-01 [PMID: 17209055]

Bioinformatics

• Gene Symbol: PIM1
• Entrez:
  • 5292(Human)
• Uniprot:
  • P11309(Human)