PIK3C2A Antibody

Immunohistochemistry-Paraffin: PIK3C2A Antibody [NB300-855] - (2ug/ml) staining of paraffin embedded Human Placenta. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

Immunohistochemistry-Paraffin: PIK3C2A Antibody [NB300-855] - IHC analysis of PIK3C2A in paraffin embedded Human Heart using NB300-855 at 5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Ca
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PIK3C2A Antibody Summary

• Description: Novus Biologicals Goat PIK3C2A Antibody (NB300-855) is a polyclonal antibody validated for use in IHC and ELISA. Anti-PIK3C2A Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-TVKWYQLTAATYL corresponding to C-Terminus according to NP_002636.2.
• Predicted Species: Mouse (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PIK3C2A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Peptide ELISA detection limit 1:32000
• Application Notes: WB: Preliminary experiments gave no signal but low background in human testis and Molt-4 lysates at up to 1 ug/ml. IHC-P: Human heart shows strong staining of the capillary network and patterned staining of the myocardial fibres in transverse section.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PIK3C2A Antibody

• C2-containing phosphatidylinositol kinase
• CPK
• DKFZp686L193
• EC 2.7.1
• EC 2.7.1.137
• EC 2.7.1.153
• EC 2.7.1.154
• MGC142218
• phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit alpha
• Phosphoinositide 3-kinase-C2-alpha
• phosphoinositide-3-kinase, class 2, alpha polypeptide
• PI3-K-C2(ALPHA)
• PI3-K-C2A
• PI3K-C2alpha
• PI3K-C2-alpha
• PIK3C2A
• PtdIns-3-kinase C2 subunit alpha

Background

PIK3C2A belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PIK3C2A Antibody (NB300-855)(1)

Publication using NB300-855

• Molz L, Chen YW, Hirano M, Williams LT. Cpk is a novel class of Drosophila PtdIns 3-kinase containing a C2 domain. J Biol Chem 1996-06-07 [PMID: 8662856]

Bioinformatics

• Gene Symbol: PIK3C2A
• Entrez:
  • 5286(Human)
  • 18704(Mouse)
• Uniprot:
  • NP_002636.(Human)