PIG3 Antibody (OTI3B11)

Western Blot: PIG3 Antibody (3B11) [NBP2-01301] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIG3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIG3.

Immunohistochemistry-Paraffin: PIG3 Antibody (3B11) [NBP2-01301] Staining of paraffin-embedded Human Kidney tissue using anti-PIG3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PIG3 Antibody (3B11) [NBP2-01301] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PIG3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PIG3 Antibody (3B11) [NBP2-01301] - Staining of paraffin-embedded Human breast tissue using anti-PIG3 mouse monoclonal antibody.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: OTI3B11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

PIG3 Antibody (OTI3B11) Summary

• Immunogen: Full length human recombinant protein of human TP53I3(NP_671713) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TP53I3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 35.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PIG3 Antibody (OTI3B11)

• p53-induced gene 3 protein
• PIG3quinone oxidoreductase homolog
• quinone oxidoreductase PIG3
• tumor protein p53 inducible protein 3
• Tumor protein p53-inducible protein 3

Background

The protein encoded by this gene is similar to oxidoreductases, which are enzymes involved in cellular responses to oxidative stresses and irradiation. This gene is induced by the tumor suppressor p53 and is thought to be involved in p53-mediated cell death. It contains a p53 consensus binding site in its promoter region and a downstream pentanucleotide microsatellite sequence. P53 has been shown to transcriptionally activate this gene by interacting with the downstream pentanucleotide microsatellite sequence. The microsatellite is polymorphic, with a varying number of pentanucleotide repeats directly correlated with the extent of transcriptional activation by p53. It has been suggested that the microsatellite polymorphism may be associated with differential susceptibility to cancer. At least two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PIG3 Antibody (NBP2-01301)(1)

Publication using NBP2-01301

• Herraiz C, Calvo F, Pandya P et al. Reactivation of p53 by a Cytoskeletal Sensor to Control the Balance Between DNA Damage and Tumor Dissemination. J Natl Cancer Inst 2016-01-01 [PMID: 26464464] (WB)

Bioinformatics

• Gene Symbol: TP53I3
• Entrez:
  • 9540(Human)