Piccolo Antibody

Western Blot: Piccolo Antibody [NBP1-54407] - Western blot analysis of Mouse, Rat brain cell lysates showing detection of Piccolo protein using Rabbit Anti-Piccolo Polyclonal Antibody (NBP1-54407). Primary Antibody: Rabbit Anti-Piccolo Polyclonal Antibody (NBP1-54407) at 1:1000.

Immunohistochemistry: Piccolo Antibody [NBP1-54407] - Immunohistochemistry analysis using Rabbit Anti-Piccolo Polyclonal Antibody (NBP1-54407). Tissue: Muscle. Species: Mouse. Primary Antibody: Rabbit Anti-Piccolo Polyclonal Antibody (NBP1-54407) at 1:400. Secondary Antibody: Alexa Fluor 488 Goat Anti-Rabbit. Counterstain: BTX (red). Localization: Selective staining of the NMJ.

Product Discontinued

Piccolo Antibody Summary

• Immunogen: Full length protein BC001304.1
• Marker: Synaptic Marker
• Specificity: No cross-reactivity to Bassoon, band at about 550 kD corresponding to the molecular weight of Piccolo, multiple isoforms can be detected.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PCLO
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000

Reactivity Notes

Human, Mouse, Rat

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4) and 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Piccolo Antibody

• ACZ
• Aczonin
• PCLO
• piccolo (presynaptic cytomatrix protein)
• Piccolo
• protein piccolo

Background

Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains. In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1 (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PCLO