Piccolo Antibody

C1qTNF3/CORS26 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Sheep Anti-Human Piccolo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7935) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Piccolo Antibody Summary

• Immunogen: E. coli-derived recombinant human Piccolo
  Ala4065-Phe4328
  Accession # NP_149015
• Specificity: Detects human Piccolo in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: PCLO
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Piccolo Antibody

• ACZ
• Aczonin
• PCLO
• piccolo (presynaptic cytomatrix protein)
• Piccolo
• protein piccolo

Background

Piccolo (PCLO; also known as Aczonin) is a 530-570 kDa member of a small family of zinc finger proteins that are associated with the cytoskeletal matrix of exocytosis-competent cells. Its name (as well as that of oboe and bassoon) derives from the assumption that this molecule is part of an ensemble of "presynaptic" proteins that orchestrate secretory events at (presynaptic) cell membranes. Piccolo is known to be initially present in the trans-Golgi of neuronal cells. When needed, it is transported to the presynaptic density (or active zones) of GABAergic and glutamergic neurons, and to the plasma membrane of pancreatic beta -cells. It reportedly forms homodimers and heterodimers with Rim2, and serves as a Ca2+ sensor for exocytosis. Human Piccolo is 5065 amino acids (aa) in length. It is a multidomain protein with N-terminal Pro- and Gln-rich sequences, two C4-type Zn-finger motifs (aa 535-1028), one PZD domain (aa 4427-4478) and two membrane-binding C2 domains (aa 4565-5057). There are at least 30 potential Thr/Ser and two Tyr phosphorylation sites. Multiple splice variants exist that principally involve insertions and deletions. None of the splice forms are missing, or show substitutions for, the range of amino acids used by R&D Systems for antibody production. Two splice forms contain identical insertions of 7, 8, 9, 17, 43 and 54 aa, and differ only in a deletion of 60 aa at the C-terminus. A third splice variant shows a 48 aa substitution for aa 443-5065, while a fourth isoform utilizes an alternative start site at Met4459 that is coupled to 1) a 17 aa insertion after Lys4570, and 2) a deletion of aa 4798-5065. Over aa 4065-4328, human Piccolo shares 97% aa sequence identity with mouse Piccolo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PCLO
• Uniprot:
  • NP_149015()