PIBF1 Antibody [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 488

PIBF1 Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human PIBF1
  Glu19-Ala419
  Accession # Q8WXW3
• Specificity: Detects human PIBF1 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PIBF1 Antibody [Alexa Fluor® 488]

• C13orf24
• chromosome 13 open reading frame 24
• KIAA1008
• PIBF
• PIBF1
• progesterone immunomodulatory binding factor 1
• progesterone-induced blocking factor 1
• progesterone-induced-blocking factor 1
• RP11-505F3.1

Background

PIBF-1 (Progesterone induced blocking factor 1) is a 90-92 kDa progesterone-inducible molecule initially isolated from the lymphocytes of pregnant women. It is produced by lymphocytes plus villous trophoblast cells, and modulates the activity of cytotoxic NK cells. Human PIBF-1 is 757 amino acids (aa) in length. It contains a possible signal sequence (aa 1-26), two Leu-zippers (aa 311-323 and 643-664), two NLSs (aa 282-285 and 567-573), a bZIP sequence (aa 574-614), and an ER retention motif (aa 752-757). PIBF-1 is found in the nucleus, cytoplasm, and circulation, the result of multiple splice variants. SDS-page shows 90 kDa, 80-83 kDa, 48-52 kDa, 34-35 kDa, and 10-12 kDa isoforms. The 90 kDa form is nuclear and full-length, while the 34-35 kDa form is secreted and likely represents aa 1-223 spliced to aa 683-757. There is a 50-55 kDa form that is apparently intracellular, possibly bioactive and likely represents the N-terminus of the molecule. Over aa 19‑419, human PIBF-1 shares 88% aa identity with mouse PIBF-1.

 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.