PIB5PA Antibody - BSA Free

Western Blot: PIB5PA Antibody [NBP2-94054] - Analysis of extracts of various cell lines, using PIB5PA at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

Immunocytochemistry/Immunofluorescence: PIB5PA Antibody [NBP2-94054] - Analysis of U2OS cells using PIB5PA . Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PIB5PA Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PIB5PA Antibody - BSA Free (NBP2-94054) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 60-360 of human INPP5J (NP_001002837.1). VTWNVGTAMPPDDVTSLLHLGGGDDSDGADMIAIGLQEVNSMLNKRLKDALFTDQWSELFMDALGPFNFVLVSSVRMQGVILLLFAKYYHLPFLRDVQTDCTRTGLGGYWGNKGGVSVRLAAFGHMLCFLNCHLPAHMDKAEQRKDNFQTILSLQQFQGPGAQGILDHDLVFWFGDLNFRIESYDLHFVKFAIDSDQLHQLWEKDQLNMAKNTWPILKGFQEGPLNFAPTFKFDVGTNKYDTSAKKRKPAWTDRILWKVKAPGGGPSPSGRKSHRLQVTQHSYRSHMEYTVSDHKPVAAQF
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: INPP5J
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:100
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for PIB5PA Antibody - BSA Free

• A
• inositol polyphosphate 5-phosphatase J
• inositol polyphosphate-5-phosphatase J
• phosphatidylinositol 4,5-bisphosphate 5-phosphatase A
• PIPP

Background

PIB5PA, also referred to as Phosphatidylinositol 4,5-bisphosphate 5-phosphatase A, has a long isoform that is 1,006 amino acids in length and 107 kDa, and two shorter isoforms that are 639 and 638 amino acids long. It is known that PIB5PA converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate, and it is thought that PIB5PA may mediate the function of plasma membrane proteins. Research is currently being conducted on PIB5PA in relation to several diseases and disorders including breast cancer, Guillain-Barre syndrome, developmental disorders and Autoimmune Lymphoproliferative Syndrome. PIB5PA has also been shown to have interactions with INPP1, PTEN, ITPK1, INPP4A and ITPKA in pathways such as lipid metabolism and lipoprotein metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: INPP5J