PIAS2 Antibody

Images

 
Immunohistochemistry-Paraffin: PIAS2 Antibody [NB100-59734] - (3.8ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PIAS2 Antibody Summary

Immunogen
Peptide with sequence KEAMKVSSQPCTKIE corresponding to internal region according to NP_775298.1, NP_004662.2.
Epitope
KEAMKVSSQPCTKIE
Specificity
This antibody is expected to recognise both reported isoforms (NP_775298.1 and NP_004662.2). No cross-reactivity expected with PIAS1, 3 and 4.
Predicted Species
Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PIAS2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: Preliminary experiments gave an approx. 90 kDa band in lysates of A431 after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 68.2 kDa band according to NP_004662.2. The 90 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human testis shows nuclear and extra-nuclear staining in spermatids.
Control
PIAS2 Overexpression Lysate
Publications
Read Publication using NB100-59734.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PIAS2 Antibody

  • Androgen receptor-interacting protein 3
  • ARIP3
  • DAB2-interacting protein
  • DIP
  • E3 SUMO-protein ligase PIAS2
  • MGC102682
  • miz
  • MIZ1
  • Msx-interacting zinc finger protein
  • Msx-interacting-zinc finger
  • PIAS2
  • PIAS-NY protein
  • PIASx alpha
  • PIASX
  • PIASX-ALPHA
  • PIASX-BETA
  • Protein inhibitor of activated STAT x
  • protein inhibitor of activated STAT, 2
  • Protein inhibitor of activated STAT2
  • SIZ2
  • zinc finger, MIZ-type containing 4
  • ZMIZ4

Background

PIAS2 functions as an E3 type small ubiquitin like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO mediated repression of ELK1 transactivation activity. Isoform PIAS2 beta, but not isoform PIAS2 alpha, promotes MDM2 sumoylation. Isoform PIAS2 beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2 alpha.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF2005
Species: Hu
Applications: CyTOF-ready, Flow, IHC, WB
NBP1-90214
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NB100-56603
Species: Ca, Hu, Mu, Pm, Rt
Applications: IHC, IHC-P, WB
MAB1455
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
664-LI/CF
Species: Hu
Applications: BA
NBP1-31302
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-89150
Species: Hu, Rt
Applications: IHC, IHC-P, WB
NBP1-98549
Species: Hu
Applications: WB
NBP1-77158
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
NBP2-41211
Species: Hu, Mu, Rt
Applications: ELISA, IF, IHC, IHC-P, WB
NBP1-47974
Species: Ca, Hu, Pm, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-89146
Species: Hu
Applications: ICC/IF, IHC, IHC-P
DY262
Species: Hu
Applications: ELISA
NBP1-57531
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-38530
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
210-TA
Species: Hu
Applications: BA
NBP2-29463
Species: Pm, Hu, Pm, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-87581
Species: Hu
Applications: IHC, IHC-P, WB
NB100-59734
Species: Hu, Mu, Rt, Ca
Applications: IHC, IHC-P, PEP-ELISA

Publications for PIAS2 Antibody (NB100-59734)(1)

Reviews for PIAS2 Antibody (NB100-59734) (0)

There are no reviews for PIAS2 Antibody (NB100-59734). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for PIAS2 Antibody (NB100-59734) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional PIAS2 Products

Bioinformatics Tool for PIAS2 Antibody (NB100-59734)

Discover related pathways, diseases and genes to PIAS2 Antibody (NB100-59734). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PIAS2 Antibody (NB100-59734)

Discover more about diseases related to PIAS2 Antibody (NB100-59734).
 

Pathways for PIAS2 Antibody (NB100-59734)

View related products by pathway.

PTMs for PIAS2 Antibody (NB100-59734)

Learn more about PTMs related to PIAS2 Antibody (NB100-59734).
 

Research Areas for PIAS2 Antibody (NB100-59734)

Find related products by research area.

Blogs on PIAS2

There are no specific blogs for PIAS2, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PIAS2 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol PIAS2
Entrez
Uniprot