Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) DuoSet IC, 2 Plate

• Reactivity: Hu, Mu, Rt
• Applications: ELISA
• Conjugate: Biotin

Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) DuoSet IC, 2 Plate Summary

• Modification: p Thr185, p Thr202, p Tyr187, p Tyr204
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) DuoSet IC, 2 Plate

• ERK1
• ERK-1
• ERK1/ERK2
• ERT2
• Extracellular signal-regulated kinase 1
• extracellular signal-related kinase 1
• HS44KDAP
• HUMKER1A
• Insulin-stimulated MAP2 kinase
• MAPK 1
• Microtubule-associated protein 2 kinase
• mitogen-activated protein kinase 3
• p44erk1
• p44-ERK1
• p44mapk
• p44-MAPK
• PRKM3

Background

ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44 and 42 kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf-ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activate both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2.

ERK5, also known as Big Mitogen-activated Protein Kinase 1 (BMK1) and MAPK7, is activated by several mechanisms, including receptor tyrosine kinases, G protein-coupled receptors, and osmotic stress. Like ERK1 and ERK2, ERK5 contains the conserved Thr-Glu-Tyr activation motif in its activation loop. Unlike these ERKs, however, ERK5 contains a unique C-terminal domain that regulates its activation and nuclear translocation.

Publications for ERK1/ERK2 (DYC1018B-2)(33)

Publications using DYC1018B-2

• Feng, S;Sanford, JA;Weber, T;Hutchinson-Bunch, CM;Dakup, PP;Paurus, VL;Attah, K;Sauro, HM;Qian, WJ;Wiley, HS; A Phosphoproteomics Data Resource for Systems-level Modeling of Kinase Signaling Networks bioRxiv : the preprint server for biology 2023-08-03 [PMID: 37577496] (Human)
• G Feigelman, E Simanovich, P Brockmeyer, MA Rahat Knocking-Down CD147/EMMPRIN Expression in CT26 Colon Carcinoma Forces the Cells into Cellular and Angiogenic Dormancy That Can Be Reversed by Interactions with Macrophages Biomedicines, 2023-03-02;11(3):. 2023-03-02 [PMID: 36979746] (Mouse)
• K Sakamoto, B Lin, K Nunomura, T Izawa, S Nakagawa The K-Ras(G12D)-inhibitory peptide KS-58 suppresses growth of murine CT26 colorectal cancer cell-derived tumors Scientific Reports, 2022-05-17;12(1):8121. 2022-05-17 [PMID: 35581303] (Mouse)
• Y Jung, M Son, YR Nam, J Choi, JR Heath, S Yang Microfluidic Single-Cell Proteomics Assay Chip: Lung Cancer Cell Line Case Study Micromachines, 2021-09-23;12(10):. 2021-09-23 [PMID: 34683198] (Human)
• A De Zutter, H Crijns, N Berghmans, M García-Cab, L Vanbrabant, N Pörtner, V Vanheule, P Verscheure, MM Siddiquei, AM Abu El-Asr, P Carmeliet, P Van Wielen, I De Meester, J Van Damme, P Proost, S Struyf The Chemokine-Based Peptide, CXCL9(74-103), Inhibits Angiogenesis by Blocking Heparan Sulfate Proteoglycan-Mediated Signaling of Multiple Endothelial Growth Factors Cancers, 2021-10-12;13(20):. 2021-10-12 [PMID: 34680238] (Human)
• K Sakamoto, T Masutani, T Hirokawa Generation of KS-58 as the first K-Ras(G12D)-inhibitory peptide presenting anti-cancer activity in vivo Scientific Reports, 2020-12-10;10(1):21671. 2020-12-10 [PMID: 33303890] (Human)
• DA Olsen, CEB Thomsen, RF Andersen, JS Madsen, A Jakobsen, I Brandslund Decreased concentrations of intracellular signaling proteins in colon cancer patients with BRAF mutations Sci Rep, 2020-11-18;10(1):20113. 2020-11-18 [PMID: 33208845] (Human)
• IA Schepetkin, LN Kirpotina, AI Khlebnikov, N Balasubram, MT Quinn Neutrophil Immunomodulatory Activity of Natural Organosulfur Compounds Molecules, 2019-05-10;24(9):. 2019-05-10 [PMID: 31083328] (Human)
• AI Khlebnikov, IA Schepetkin, AS Kishkentae, ZR Shaimerden, GA Atazhanova, SM Adekenov, LN Kirpotina, MT Quinn Inhibition of T Cell Receptor Activation by Semi-Synthetic Sesquiterpene Lactone Derivatives and Molecular Modeling of Their Interaction with Glutathione and Tyrosine Kinase ZAP-70 Molecules, 2019-01-19;24(2):. 2019-01-19 [PMID: 30669433] (Human)
• S Bhattachar, L Feferman, G Sharma, JK Tobacman Increased GPNMB, phospho-ERK1/2, and MMP-9 in cystic fibrosis in association with reduced arylsulfatase B Mol. Genet. Metab., 2018-02-20;0(0):. 2018-02-20 [PMID: 29703589] (Human)
• B Marcus-Sam, CC Krieger, A Boutin, G Kahaly, S Neumann, MC Gershengor Evidence that Graves' Ophthalmopathy Immunoglobulins Do Not Directly Activate IGF-1 Receptors Thyroid, 2018-04-30;0(0):. 2018-04-30 [PMID: 29631510] (Human)
• Jong-Sup Bae Anti-vascular inflammatory effects of pentacyclic triterpenoids from Astilbe rivularis in vitro and in vivo Chem. Biol. Interact., 2016-11-22;261(0):127-138. 2016-11-22 [PMID: 27887929] (Human)
• Joerg Mattes TRAIL signaling is pro-inflammatory and pro-viral in a murine model of rhinovirus 1B infection Am. J. Physiol. Lung Cell Mol. Physiol., 2016-11-11;0(0):ajplung.00200. 2016-11-11 [PMID: 27836899] (Mouse)
• Combining nonclinical experiments with translational PKPD modeling to differentiate erlotinib and gefitinib Mol Cancer Ther, 2016-09-16;0(0):. 2016-09-16 [PMID: 27638857] (Mouse)
• R Janssens, A Mortier, D Boff, V Vanheule, M Gouwy, C Franck, O Larsen, MM Rosenkilde, J Van Damme, FA Amaral, MM Teixeira, S Struyf, P Proost Natural nitration of CXCL12 reduces its signaling capacity and chemotactic activity in vitro and abrogates intra-articular lymphocyte recruitment in vivo Oncotarget, 2016-09-20;7(38):62439-62459. 2016-09-20 [PMID: 27566567] (Mouse)
• Knoop , K A, McDonald , K G, McCrate , S, McDole , J R, Newberry , R D Microbial sensing by goblet cells controls immune surveillance of luminal antigens in the colon. Mucosal Immunol, 2014-07-09;8(1):198-210. 2014-07-09 [PMID: 25005358] (Human)
• Ghezali L, Liagre B, Limami Y, Beneytout J, Leger D Sonic Hedgehog activation is implicated in diosgenin-induced megakaryocytic differentiation of human erythroleukemia cells. PLoS ONE, 2014-04-16;9(4):e95016. 2014-04-16 [PMID: 24740159] (Human)
• Summers L, Kangwantas K, Rodriguez-Grande B, Denes A, Penny J, Kielty C, Pinteaux E Activation of brain endothelial cells by interleukin-1 is regulated by the extracellular matrix after acute brain injury. Mol Cell Neurosci, 2013-10-24;57(0):93-103. 2013-10-24 [PMID: 24161715] (Rat)
• Ghezali L, Leger D, Limami Y, Cook-Moreau J, Beneytout J, Liagre B Cyclopamine and jervine induce COX-2 overexpression in human erythroleukemia cells but only cyclopamine has a pro-apoptotic effect. Exp Cell Res, 2013-01-25;319(7):1043-53. 2013-01-25 [PMID: 23357584] (Human)
• Vernitsky H, Rechavi O, Rainy N, Besser M, Nagar M, Schachter J, Lerenthal Y, Ehrlich M, Kloog Y, Goldstein I Ras oncoproteins transfer from melanoma cells to T cells and modulate their effector functions. J Immunol, 2012-10-01;189(9):4361-70. 2012-10-01 [PMID: 23028055] (Human)
• Gouwy M, Struyf S, Berghmans N, Vanormelingen C, Schols D, Van Damme J CXCR4 and CCR5 ligands cooperate in monocyte and lymphocyte migration and in inhibition of dual-tropic (R5/X4) HIV-1 infection. Eur. J. Immunol., 2011-03-07;41(4):963-73. 2011-03-07 [PMID: 21381021] (Human)
• Chen J, Olivares-Navarrete R, Wang Y Protein-disulfide isomerase-associated 3 (Pdia3) mediates the membrane response to 1,25-dihydroxyvitamin D3 in osteoblasts. J. Biol. Chem., 2010-09-15;285(47):37041-50. 2010-09-15 [PMID: 20843786] (Mouse)
• Lorquet S, Berndt S, Blacher S, Gengoux E, Peulen O, Maquoi E, Noel A, Foidart JM, Munaut C, Pequeux C Soluble forms of VEGF receptor-1 and -2 promote vascular maturation via mural cell recruitment. FASEB J., 2010-05-19;24(10):3782-95. 2010-05-19 [PMID: 20484670] (Human)
• Yeh CC, Li H, Malhotra D, Turcato S, Nicholas S, Tu R, Zhu BQ, Cha J, Swigart PM, Myagmar BE, Baker AJ, Simpson PC, Mann MJ Distinctive ERK and p38 signaling in remote and infarcted myocardium during post-MI remodeling in the mouse. J. Cell. Biochem., 2010-04-15;109(6):1185-91. 2010-04-15 [PMID: 20186881] (Mouse)
• Wang S, Zhou Q, Gallo JM Demonstration of the equivalent pharmacokinetic/pharmacodynamic dosing strategy in a multiple-dose study of gefitinib. Mol. Cancer Ther., 2009-06-09;8(6):1438-47. 2009-06-09 [PMID: 19509243] (Human)
• Struyf S, Noppen S, Loos T, Mortier A, Gouwy M, Verbeke H, Huskens D, Luangsay S, Parmentier M, Geboes K, Schols D, Van Damme J, Proost P Citrullination of CXCL12 differentially reduces CXCR4 and CXCR7 binding with loss of inflammatory and anti-HIV-1 activity via CXCR4. J. Immunol., 2009-01-01;182(1):666-74. 2009-01-01 [PMID: 19109200] (Human)
• Loos T, Mortier A, Gouwy M, Ronsse I, Put W, Lenaerts JP, Van Damme J, Proost P Citrullination of CXCL10 and CXCL11 by peptidylarginine deiminase: a naturally occurring posttranslational modification of chemokines and new dimension of immunoregulation. Blood, 2008-07-21;112(7):2648-56. 2008-07-21 [PMID: 18645041] (Hamster)
• Gouwy M, Struyf S, Noppen S, Schutyser E, Springael JY, Parmentier M, Proost P, Van Damme J Synergy between coproduced CC and CXC chemokines in monocyte chemotaxis through receptor-mediated events. Mol. Pharmacol., 2008-05-09;74(2):485-95. 2008-05-09 [PMID: 18469140] (Hamster, Human)
• Aguila B, Coulbault L, Boulouard M, Leveille F, Davis A, Toth G, Borsodi A, Balboni G, Salvadori S, Jauzac P, Allouche S In vitro and in vivo pharmacological profile of UFP-512, a novel selective delta-opioid receptor agonist; correlations between desensitization and tolerance. Br. J. Pharmacol., 2007-11-05;152(8):1312-24. 2007-11-05 [PMID: 17982482] (Human)
• Muselet-Charlier C, Roque T, Boncoeur E, Chadelat K, Clement A, Jacquot J, Tabary O Enhanced IL-1beta-induced IL-8 production in cystic fibrosis lung epithelial cells is dependent of both mitogen-activated protein kinases and NF-kappaB signaling. Biochem. Biophys. Res. Commun., 2007-04-02;357(2):402-7. 2007-04-02 [PMID: 17420005] (Human)
• Leger DY, Liagre B, Beneytout JL Low dose leflunomide activates PI3K/Akt signalling in erythroleukemia cells and reduces apoptosis induced by anticancer agents. Apoptosis, 2006-10-01;11(10):1747-60. 2006-10-01 [PMID: 16927021] (Human)
• Gee K, Angel JB, Ma W, Mishra S, Gajanayaka N, Parato K, Kumar A Intracellular HIV-Tat expression induces IL-10 synthesis by the CREB-1 transcription factor through Ser133 phosphorylation and its regulation by the ERK1/2 MAPK in human monocytic cells. J. Biol. Chem., 2006-08-18;281(42):31647-58. 2006-08-18 [PMID: 16920714] (Human)
• Vergne-Salle</LastName><F P</Initial, Vergne-Salle P, Leger DY, Bertin P, Treves R, Beneytout JL, Liagre B Effects of the active metabolite of leflunomide, A77 1726, on cytokine release and the MAPK signalling pathway in human rheumatoid arthritis synoviocytes. Cytokine, 2005-09-07;31(5):335-48. 2005-09-07 [PMID: 16099671] (Human)