PHLPP2 Antibody

Western Blot: PHLPP2 Antibody [NBP2-19803] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 5% SDS PAGE gel, diluted at 1:2000.

Immunocytochemistry/Immunofluorescence: PHLPP2 Antibody [NBP2-19803] - Analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PHLPP2 Antibody Summary

• Description: Novus Biologicals Rabbit PHLPP2 Antibody (NBP2-19803) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human PHLPP2. The exact sequence is proprietary.
• Localization: Cytoplasm; Membrane; Peripheral membrane protein; Nucleus
• Predicted Species: Rat (97%), Bovine (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PHLPP2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 147 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for PHLPP2 Antibody

• EC 3.1.3.16
• KIAA0931PH domain leucine-rich repeat-containing protein phosphatase 2
• PH domain and leucine rich repeat protein phosphatase 2
• PH domain and leucine rich repeat protein phosphatase-like
• PH domain leucine-rich repeat-containing protein phosphatase-like
• PHLPPLPHLPP-like

Background

PHLPP2 contains 21 LRR (leucine rich) repeats, 1 PH domain and 1 PP2C like domain. It is a protein phosphatase that specifically mediates dephosphorylation of AKT1 Ser 473, a protein that regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro and antiapoptotic genes. Dephosphorylation of Ser 473 of AKT1 triggers apoptosis and decrease cell proliferation. PHLPP2 also controls the phosphorylation of AKT3. It binds 2 manganese ions per subunit. There are 3 named isoforms produced by alternative splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PHLPP2
• Uniprot:
  • Q6ZVD8(Human)