PHLPP2 Antibody

Western Blot: PHLPP2 Antibody [NB100-1812] - Whole cell lysate (50 ug) from HeLa, RKO, MCF-7, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-PHLPPL antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

PHLPP2 Antibody [NB100-1812] - Detection of human PHLPPL by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-PHLPPL antibody NB100-1812 (lot NB100-1812-2) used for IP at 6 ug per reaction. PHLPPL was also immunoprecipitated by a previous lot of this antibody (lot NB100-1812-1). For blotting immunoprecipitated PHLPPL, NB100-1812 was used at 0.04 ug/ml. Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

PHLPP2 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 1275 and the C-terminus (residue 1323) of human PH Domain and Leucine Rich Repeat Protein Phosphatase-like using the numbering given in entry NP_055835.2 (GeneID 23035).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PHLPP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 ug/ml
  • Western Blot 1:2000-1:10000

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 23993956).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PHLPP2 Antibody

• EC 3.1.3.16
• KIAA0931PH domain leucine-rich repeat-containing protein phosphatase 2
• PH domain and leucine rich repeat protein phosphatase 2
• PH domain and leucine rich repeat protein phosphatase-like
• PH domain leucine-rich repeat-containing protein phosphatase-like
• PHLPPLPHLPP-like

Background

PHLPPL (PH domain leucine-rich repeat-containing protein phosphatase-like) is responsible for the dephosphorylation and subsequent inactivation of Akt. Akt is a signaling protein critical to the balance of cell survival and apoptosis. Dephosphorylation of Akt by PHLPPL has been demonstrated to trigger apoptosis and suppress tumor cell growth. PHLPPL is also known as PHLPPL2 and functions similarly to its relative, PHLPP1. Recent studies show that PHLPPL and PHLPP selectively terminate Akt-signaling by targeting specific Akt isoforms. Alternate names for PHLPPL include PH Domain and Leucine Rich Repeat Protein Phosphatase-Like, KIAA0931, and PHLPP2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PHLPP2 Antibody (NB100-1812)(2)

Publications using NB100-1812

• Rajala A, Gupta VK, Anderson RE, Rajala RV. Light activation of the insulin receptor regulates mitochondrial hexokinase. A possible mechanism of retinal neuroprotection. Mitochondrion. 2013-08-30 [PMID: 23993956] (WB, Rat)
• Sangai T, Akcakanat A, Chen H et al. Biomarkers of response to Akt inhibitor MK-2206 in breast cancer. Clin Cancer Res 2012-10-01 [PMID: 22932669]

Bioinformatics

• Gene Symbol: PHLPP2
• Entrez:
  • 23035(Human)
  • 244650(Mouse)
• Uniprot:
  • Q6ZVD8(Human)