PHIP Antibody (4D7) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: PHIP Antibody (4D7) [H00055023-M01] - Analysis of monoclonal antibody to PHIP on HeLa cell . Antibody concentration 10 ug/ml.

ELISA: PHIP Antibody (4D7) [H00055023-M01] - Detection limit for recombinant GST tagged PHIP is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 4D7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PHIP Antibody (4D7) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PHIP Antibody (4D7) - Azide and BSA Free (H00055023-M01) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-PHIP Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: PHIP (NP_060404, 1599 a.a. ~ 1705 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TLSKSSAVIEQGDCKNNALVPGTIQVNGHGGQPSKLVKRGPGRKPKVEVNTNSGEIIHKKRGRKPKKLQYAKPEDLEQNNVHPIRDEVLPSSTCNFLSETNNVKEDL
• Specificity: PHIP - pleckstrin homology domain interacting protein
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PHIP
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. It is also useful for immunofluorescence.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PHIP Antibody (4D7) - Azide and BSA Free

• DCAF14
• DDB1 and CUL4 associated factor 14
• FLJ20705
• FLJ45918
• IRS-1 PH domain-binding protein
• MGC90216
• ndrp
• PH-interacting protein
• pleckstrin homology domain interacting protein
• WD repeat-containing protein 11
• WDR11

Background

PH-interacting protein (PHIP) was isolated as factor that binds to the pleckstrin homology (PH) domain of insulin receptor substrate-1 (IRS-1). PHIP was determined not to be a substrate of the insulin receptor but may instead serve to link IRS-1 to the insulin receptor. PHIP has also been implicated as a positive regulator of beta-cell mitogenesis and survival. PHIP is also known as WD-repeat-containing protein 11, WDR11, and IRS-1 PH domain-binding protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PHIP Antibody (H00055023-M01)(2)

Publications using H00055023-M01

• De Semir D, Nosrati M, Bezrookove V et al. Pleckstrin homology domain-interacting protein (PHIP) as a marker and mediator of melanoma metastasis. Proc Natl Acad Sci U S A. 2012-04-17 [PMID: 22511720]
• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01

Bioinformatics

• Gene Symbol: PHIP
• Entrez:
  • 55023(Human)
• Uniprot:
  • NP_060404(Human)