PHGDH Antibody (CL0555) - Azide and BSA Free

Analysis in human pancreas and skeletal muscle tissues using NBP3-43966 antibody. Corresponding PHGDH RNA-seq data are presented for the same tissues.

Lane 1: Marker [kDa]Lane 2: Human cell line RT-4

Lane 1: Marker [kDa]Lane 2: Human cell line HeLa cytoplasmic fractionLane 3: Human cell line HeLa membrane fractionLane 4: Human cell line HeLa nuclear fractionLane 5: Human cell line HeLa chromatin fractionLane 6: Human cell line HeLa cytoskeletal fraction

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Staining of MCF7 cells using the Anti-PHGDH monoclonal antibody) .

Staining of human skin shows strong cytoplasmic positivity in squamous epithelial cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL0555
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

PHGDH Antibody (CL0555) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PHGDH Antibody (CL0555) - Azide and BSA Free (NBP2-52939) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of O43175, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PHGDH
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for PHGDH Antibody (CL0555) - Azide and BSA Free

• 3,3-phosphoglycerate dehydrogenase
• 3PGDH
• 3-PGDH
• D-3-phosphoglycerate dehydrogenase
• EC 1.1.1
• EC 1.1.1.95
• MGC3017
• PDG
• PDGH3
• PGAD
• PGD
• PGDH
• PHGDH
• phosphoglycerate dehydrogenase
• SERA

Background

3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PHGDH