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PHF8 Antibody

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Western Blot: PHF8 Antibody [NB100-93313] - Detection of human and mouse PHF8 by western blot. Samples: Whole cell lysate (15 and 50 ug) from Jurkat, HeLa, HEK293T, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHF8 antibody NB100-93313 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Western Blot: PHF8 Antibody [NB100-93313] - Detection of human and mouse PHF8 by western blot. Samples: Whole cell lysate (15 and 50 ug) from Jurkat, HeLa, HEK293T, and NIH 3T3 cells prepared using NETN lysis buffer. ...read more
Immunohistochemistry-Paraffin: PHF8 Antibody [NB100-93313] - Section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- PHF8 used at a dilution of 1:200 (1 ug/ml). Detection: DAB
Independent Antibodies: Western Blot: PHF8 Antibody [NB100-93313] - Detection of Human PHF8 on HeLa whole cell lysate using NB100-93313. PHF8 was also immunoprecipitated by rabbit anti-PHF8 antibody NB100-93314.
Immunohistochemistry-Paraffin: PHF8 Antibody [NB100-93313] - Section of human small cell lung cancer. Antibody: Affinity purified rabbit anti- PHF8 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP, CHIP-SEQ
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
 

Independent Antibodies

        

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PHF8 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 650 and 700 of human PHD finger protein 8 (CAI41576.1).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PHF8
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation Sequencing
  • Immunohistochemistry 1:100- 1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
ChIP-Seq 10 ug as per Ram O et al., Cell. 2011 Dec 23;147(7):1628-39. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Publications
Read Publications using
NB100-93313 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PHF8 Antibody

  • EC 1.14.11.27
  • histone lysine demethylase PHF8
  • jumonji C domain-containing histone demethylase 1F
  • KIAA1111
  • KIAA1111ZNF422JHDM1F
  • MRXSSD
  • PHD finger protein 8DKFZp686E0868
  • PHF8
  • ZNF422

Background

PHF8 (PHD finger protein 8) is a member of the jumonji family of proteins and contains a jumonji C (JmjC) domain. The JmjC proteins are predicted to be metalloenzymes that play a role a chromatin remodeling and histone demethylation. PHF8 also bears a PHD (plant homeodomain)- type zinc-finger, a domain also found to be involved in chromatin remodeling and transcriptional regulation. Truncating mutations in PHF8 are associated with X-linked mental retardation and cleft lip/cleft palate. PHF8 is also known as ZNF422 and KIAA1111.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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PHF8 Antibody
NB100-93313
PHF8 Antibody
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP, CHIP-SEQ

Publications for PHF8 Antibody (NB100-93313)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


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Bioinformatics

Gene Symbol PHF8
Entrez
Uniprot