Recombinant Human PGPEP-1 Protein

Product Discontinued

Recombinant Human PGPEP-1 Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-209 of Human PGPEP-1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMEQPRKA VVVTGFGPFG EHTVNASWIA VQELEKLGLG DSVDLHVYEI PVEYQTVQRL IPALWEKHSP QLVVHVGVSG MATTVTLEKC GHNKGYKGLD NCRFCPGSQC CVEDGPESID SIIDMDAVCK RVTTLGLDVS VTISQDAGRY LCDFTYYTSL YQSHGRSAFV HVPPLGKPYN ADQLGRALRA IIEEMLDLLE QSEGKINYCH KH

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PGPEP1
• Purity: >95% pure by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 25.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 20% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95% pure by SDS-PAGE

Alternate Names for Recombinant Human PGPEP-1 Protein

• EC 3.4.19.3
• PAP-I
• PCP
• PGPEP1
• PGPEP-1
• PGPIFLJ20208
• PGP-IPAP-I
• PGPMGC10812
• Pyroglutamyl aminopeptidase I
• pyroglutamyl-peptidase 1
• pyroglutamyl-peptidase I5-oxoprolyl-peptidase
• Pyrrolidone-carboxylate peptidase

Background

PGPEP1 is an omega peptidase which removes pyroglutamyl residues from the amino termini of peptides and proteins. It is a cytosolic cysteine peptidase that is expressed in most cell types. The enzyme requires a thiolreducing agent for activity. PGPEP1 is potentially involved in the inactivation of biologically active peptides that possess an amino terminal pyroglutamyl group. Examples of such peptides include neurotensin, luteinizing hormone releasing hormone, and thyrotropinreleasing hormone. Recombinant human PGPEP1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PGPEP1