PGLYRP1/PGRP-S Antibody

Western Blot: PGLYRP1/PGRP-S Antibody [NBP1-20962] - Staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: PGLYRP1/PGRP-S Antibody [NBP1-20962] - Human spleen. Image submitted by a verified customer review.

Immunohistochemistry-Paraffin: PGLYRP1/PGRP-S Antibody [NBP1-20962] - Staining of Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PGLYRP1/PGRP-S Antibody Summary

• Immunogen: Peptide with sequence C-PGNQLYHLIQN corresponding to C-Terminus according to NP_005082.1.
• Epitope: C-PGNQLYHLIQN
• Predicted Species: Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PGLYRP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 30 kDa band observed in Jurkat lysates is reported in scientific literature (PMID: 16354652). IHC-P: Human spleen shows staining of select splenocytes.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PGLYRP1/PGRP-S Antibody

• peptidoglycan recognition protein 1
• Peptidoglycan recognition protein short
• PGLYRP
• PGLYRP1
• PGRPpeptidoglycan recognition protein
• PGRPS
• PGRP-S
• PGRP-SMGC126894
• PGRPSMGC126896
• TAG7
• TNF superfamily, member 3 (LTB)-like (peptidoglycan recognition protein)
• TNFSF3L

Background

The primary immune recognition is based on structures common among invading pathogens. Bacterial surface molecules, such as lipopolysaccharide (LPS) and peptidoglycan (PGN), are known to elicit immune reactions ranging from cytokine release to fever. Recently, a family of proteins called peptidoglycan recognition protein (PGRP) has been identified in mouse and human that binds to peptidoglycans expressed on Gram-positive bacteria. Peptidoglycan (PGN) is an essential cell wall component of virtually all bacteria (1,2) and, thus, it is an excellent target for recognition by the eukaryotic innate immune system. The PGRPs (PGRP-L, PGRP-S, PGRP-Ia, and PGRP-Ib) define a new family of human pattern recognition molecules (3). PGRP-L is primarily expressed in the liver. Although liver is not considered a primary immune organ, liver participates in host defenses by producing acute phase proteins (by hepatocytes) in response to infections and by clearing microorganisms from blood (4). PGRP-S is present in neutrophils and inhibits growth of Gram-positive bacteria and, therefore, may function as a neutrophil antibacterial protein (5). However, PGRP-S may have another, as yet unidentified function because in humans it is expressed in the bone marrow 50

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PGLYRP1/PGRP-S Antibody (NBP1-20962)(2)

Publications using NBP1-20962

• Bhusal, A;Kim, JH;Kim, SC;Hwang, EM;Ryu, H;Ali, MS;Park, SC;Lee, WH;Suk, K; The microglial innate immune protein PGLYRP1 mediates neuroinflammation and consequent behavioral changes Cell reports 2024-02-22 [PMID: 38393947]
• Lu X, Wang M, Qi J et al. Peptidoglycan recognition proteins are a new class of human bactericidal proteins. J Biol Chem 2006-03-01 [PMID: 16354652]

Review for PGLYRP1/PGRP-S Antibody (NBP1-20962) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Carol Bain IHC-P Human 05/29/2014

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human spleen, FFPE
• Species: Human

Bioinformatics

• Gene Symbol: PGLYRP1
• Entrez:
  • 8993(Human)
• Uniprot:
  • NP_005082.1(Human)