PGAM5 Antibody (CL0624) - Azide and BSA Free

Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.

Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.

Staining of human tonsil shows strong granular cytoplasmic positivity in germinal center cells.

Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PGAM5 antibody. Remaining relative intensity is presented. Loading control: Anti-PARP1.

Staining of human testis shows moderate to strong granular cytoplasmic positivity in cells in seminiferous ducts.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL0624
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

PGAM5 Antibody (CL0624) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PGAM5 Antibody (CL0624) - Azide and BSA Free (NBP2-52947) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of Q96HS1, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PGAM5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for PGAM5 Antibody (CL0624) - Azide and BSA Free

• Bcl-XL-binding protein v68
• BXLBv68
• EC 3.1.3.16
• MGC5352
• phosphoglycerate mutase family member 5BXLBV68
• serine/threonine-protein phosphatase PGAM5, mitochondrial

Background

Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate fora Keap1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2-dependent gene expression

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PGAM5