PFKFB4 Antibody (OTI1C8)

Western Blot: PFKFB4 Antibody (1C8) [NBP2-02226] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PFKFB4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PFKFB4.

Immunocytochemistry/Immunofluorescence: PFKFB4 Antibody (1C8) [NBP2-02226] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PFKFB4.

Immunocytochemistry/Immunofluorescence: PFKFB4 Antibody (1C8) [NBP2-02226] - Staining of HepG2 cells using anti-PFKFB4 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: OTI1C8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.9 mg/ml

PFKFB4 Antibody (OTI1C8) Summary

• Description: Novus Biologicals Mouse PFKFB4 Antibody (OTI1C8) (NBP2-02226) is a monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human PFKFB4 (NP_004558) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PFKFB4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.9 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PFKFB4 Antibody (OTI1C8)

• 6PF-2-K/Fru-2,6-P2ase 4
• 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4,6-phosphofructo-2-kinase/fructose-2,6-biphosphatase-4,6PF-2-K/Fru-2,6-P2ase 4,6PF-2-K/Fru-2,6-P2ase testis-type isozyme
• bifunctional enzyme with kinase and biphosphatase activities
• PFK/FBPase 4
• PFKFB4

Background

Synthesis and degradation of fructose 2,6-bisphosphate

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PFKFB4
• Entrez:
  • 829(Human)