Peroxiredoxin 5 Antibody [Alexa Fluor® 700]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 700

Peroxiredoxin 5 Antibody [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant mouse Peroxiredoxin 5
  Met1-Leu210
  Accession # P99029
• Specificity: Detects human, mouse and rat Peroxiredoxin 5 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Peroxiredoxin 5 Antibody [Alexa Fluor® 700]

• ACR1
• ACR1Peroxisomal antioxidant enzyme
• Alu corepressor 1
• Alu co-repressor 1
• Antioxidant enzyme B166
• AOEB166
• AOEB166Peroxiredoxin V
• AOPP
• B166
• EC 1.11.1.15
• Liver tissue 2D-page spot 71B
• MGC117264
• MGC142283
• MGC142285
• Peroxiredoxin 5
• peroxiredoxin-5, mitochondrial
• PLPThioredoxin peroxidase PMP20
• PMP20
• PRDX5
• PRDX6
• PRXV
• prx-V
• SBBI10
• Thioredoxin reductase
• TPx type VI

Background

Peroxiredoxin 5 (Prx-5; also known as AOEB166 and thioredoxin reductase) is a 22 kDa, widely expressed mitochondrial antioxidant enzyme that belongs to the atypical 2-Cys class of the TSA/ahpC family of peroxiredoxins. The mouse precursor molecule is 210 amino acids (aa) in length and contains an N-terminal 48 aa mitochondrial targeting (signal) sequence and a 162 aa mature enzyme that shows an NES between aa 54‑63, and a peroxisome targeting motif at aa 208‑210. There are two catalytic cysteines, one at Cys96, and another at Cys200 of the precursor. Prx-5 is a monomer that is found in mitochondria, cytosol, nucleus and peroxisomes. It is known to reduce H₂O₂ and alkyl hydroperoxides. Two potential splice forms are reported. One is 17 kDa in size and shows an alternative start site at Met49, while a second shows a 19 aa substitution for aa 83‑102. Mouse Prx-5 is 78% and 90% aa identical to human and rat Prx-5, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.