Peroxiredoxin 3 Antibody (1E3-B2) - Azide and BSA Free

Western Blot: Peroxiredoxin 3 Antibody (1E3-B2) [H00010935-M01] - Analysis of PRDX3 expression in transfected 293T cell line by PRDX3 monoclonal antibody (M01), clone 1E3-B2.Lane 1: PRDX3 transfected lysate (Predicted MW: 27.7 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: Peroxiredoxin 3 Antibody (1E3-B2) [H00010935-M01] - Analysis of monoclonal antibody to PRDX3 on HeLa cell . Antibody concentration 10 ug/ml.

Immunoprecipitation: Peroxiredoxin 3 Antibody (1E3-B2) [H00010935-M01] - Analysis of PRDX3 transfected lysate using anti-PRDX3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PRDX3 MaxPab rabbit polyclonal antibody.

ELISA: Peroxiredoxin 3 Antibody (1E3-B2) [H00010935-M01] - Detection limit for recombinant GST tagged PRDX3 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IP
• Clone: 1E3-B2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Peroxiredoxin 3 Antibody (1E3-B2) - Azide and BSA Free Summary

• Immunogen: PRDX3 (AAH02685, 1 a.a. ~ 256 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAAVGRLLRASVARHVSAIPWGISATAALRPAACGRTSLTNLLCSGSSQAKLFSTSSSCHAPAVTQHAPYFKGTAVVNGEFKDLSLDDFKGKYLVLFFYPLDFTFVCPTEIVAFSDKANEFHDVNCEVVAVSVDSHFSHLAWINTPRKNGGLGHMNIALLSDLTKQISRDYGVLLEGSGLALRGLFIIDPNGVIKHLSVNDLPVGRSVEETLRLVKAFQYVETHGEVCPANWTPDSPTIKPSPAASKEYFQKVNQ
• Specificity: PRDX3 - peroxiredoxin 3
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRDX3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot
• Application Notes: Antibody reactive against recombinant protein with GST tag on ELISA and immunofluorescence. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Peroxiredoxin 3 Antibody (1E3-B2) - Azide and BSA Free

• Antioxidant protein 1MGC104387
• AOP-1
• AOP-1MGC24293
• AOP1PRO1748
• EC 1.11.1.15
• HBC189
• MER5
• Peroxiredoxin 3
• Peroxiredoxin III
• peroxiredoxin-3
• PRDX3
• Protein MER5 homolog
• prx-III
• SP-22
• thioredoxin-dependent peroxide reductase, mitochondrial

Background

This gene encodes a protein with antioxidant function and is localized in the mitochondrion. This gene shows significant nucleotide sequence similarity to the gene coding for the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase. Expression of this gene product in E. coli deficient in the C22-subunit gene rescued resistance of the bacteria to alkylhydroperoxide. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologues suggest that these genes consist of a family that is responsible for regulation of cellular proliferation, differentiation, and antioxidant functions. Two transcript variants encoding two different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Peroxiredoxin 3 Antibody (H00010935-M01)(1)

Publication using H00010935-M01

• Tang Z, Baykal AT, Gao H et al. mTor Is a Signaling Hub in Cell Survival: A Mass-Spectrometry-Based Proteomics Investigation. J. Proteome Res. 2014-04-14 [PMID: 24694195] (WB, Human)

Bioinformatics

• Gene Symbol: PRDX3
• Entrez:
  • 10935(Human)
• OMIM:
  • 604769(Human)
• Uniprot:
  • AAH02685(Human)