Human Perforin ELISA Kit

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ELISA: Perforin ELISA kit [NBP3-11726] - Representative standard curve.

Product Details

Summary
Product Discontinued
View other related Perforin ELISA Kits

Order Details


    • Catalog Number
      NBP3-11726
    • Availability
      Product Discontinued

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Human Perforin ELISA Kit Summary

Standard Curve Range
30 - 10000 pg/mL
Sensitivity
15 pg/mL
Inter-Assay
%CV < 4.0
Intra-Assay
%CV < 2.8
Sample Volume
100 uL
Kit Type
ELISA Kit
Gene
PRF1

Applications/Dilutions

Dilutions
  • ELISA
Publications
Read Publication using NBP3-11726.

Reactivity Notes

Human and non-human primates

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated detection mAb (Pf-344)
  2. Buffers for washing and dilution and standard reconstitution
  3. Human Perforin ELISA standard
  4. Pre-coated ELISA strip plates (mAbs Pf-80/164)
  5. Streptavidin-HRP
  6. Substrate (TMB)

Alternate Names for Human Perforin ELISA Kit

  • Cytolysin
  • FLH2
  • HPLH2
  • HPLH2lymphocyte pore forming protein
  • Lymphocyte pore-forming protein
  • MGC65093
  • P1
  • P1PFN1
  • perforin 1 (pore forming protein)
  • Perforin
  • perforin-1
  • PFP
  • PFPcytolysin
  • PRF1

Background

Perforin (PRF1) is a 70 kD cytolytic protein that is the major protein constituent of cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Specifically, perforin enters the target cell's plasma membrand and creates a intercellular pore to mediate targeted cell lysis. PRF1 antibodies are useful tools for lysis research on both CTLs and NK cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for Perforin ELISA Kit (NBP3-11726)(1)

We have publications tested in 1 application: ELISA.


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Perforin ELISA Kit (NBP3-11726). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Additional Perforin Products

Research Areas for Perforin ELISA Kit (NBP3-11726)

Find related products by research area.

Blogs on Perforin.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

Perforin a Protective Serial Killer
Secretory granule-mediated cell death is one of the the key mechanisms for elimination of virus-infected and transformed target cells by cytotoxic lymphocytes. Formation of the immunological synapse between an effector and a target cell leads to exocy...  Read full blog post.

Perforin Antibodies Reveal Links to Apoptosis and Immune Response
Perforin, also known as the pore-forming protein, pfp, is a 70 kD cytolytic protein expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It is one of the major effector molecules used by CTL and NK cel...  Read full blog post.

Perforin Antibodies for Detecting Immune System Diseases
 Perforin is a calcium-dependent pore forming cytolytic protein. Perforin is partially homologous to the terminal components of the membrane attack complex of complement and produces pores of up to 20nm in diameter on target membranes. Killer lymphocy...  Read full blog post.

Perforin Antibodies Reveal Links to Apoptosis and Immune Response
Perforin, also known as the pore-forming protein, pfp, is a 70 kD cytolytic protein expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It is one of the major effector molecules used by CTL and NK cel...  Read full blog post.

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Bioinformatics

Gene Symbol PRF1