Mouse Perforin ELISA Kit (Colorimetric)

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ELISA: Mouse Perforin ELISA Kit (Colorimetric) [NBP3-00452] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.09 ng/mL

Order Details

Mouse Perforin ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse Perforin in samples. No significant cross-reactivity or interference between Mouse Perforin and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.09 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
5.59%
Intra-Assay
5.32%
Spike Recovery
88 - 108%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
PRF1

Packaging, Storage & Formulations

Storage
Storage is content dependent.

Kit Components

Components
  1. Substrate Reagent
  2. Stop Solution
  3. Biotinylated Detection Ab Diluent
  4. Concentrated Biotinylated Detection Ab (100x)
  5. Certificate of Analysis
  6. Concentrated Wash Buffer (25x)
  7. Concentrated HRP Conjugate (100x)
  8. HRP Conjugate Diluent
  9. Micro ELISA Plate(Dismountable)
  10. Reference Standard & Sample Diluent
  11. Reference Standard
  12. Plate Sealer
  13. Product Description

Alternate Names for Mouse Perforin ELISA Kit (Colorimetric)

  • Cytolysin
  • FLH2
  • HPLH2
  • HPLH2lymphocyte pore forming protein
  • Lymphocyte pore-forming protein
  • MGC65093
  • P1
  • P1PFN1
  • perforin 1 (pore forming protein)
  • Perforin
  • perforin-1
  • PFP
  • PFPcytolysin
  • PRF1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Blogs on Perforin.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

Perforin a Protective Serial Killer
Secretory granule-mediated cell death is one of the the key mechanisms for elimination of virus-infected and transformed target cells by cytotoxic lymphocytes. Formation of the immunological synapse between an effector and a target cell leads to exocy...  Read full blog post.

Perforin Antibodies Reveal Links to Apoptosis and Immune Response
Perforin, also known as the pore-forming protein, pfp, is a 70 kD cytolytic protein expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It is one of the major effector molecules used by CTL and NK cel...  Read full blog post.

Perforin Antibodies for Detecting Immune System Diseases
 Perforin is a calcium-dependent pore forming cytolytic protein. Perforin is partially homologous to the terminal components of the membrane attack complex of complement and produces pores of up to 20nm in diameter on target membranes. Killer lymphocy...  Read full blog post.

Perforin Antibodies Reveal Links to Apoptosis and Immune Response
Perforin, also known as the pore-forming protein, pfp, is a 70 kD cytolytic protein expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It is one of the major effector molecules used by CTL and NK cel...  Read full blog post.

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Bioinformatics

Gene Symbol PRF1