Perforin Antibody Pair

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Summary
Product Discontinued
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    • Catalog Number
      H00005551-PW2
    • Availability
      Product Discontinued

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Perforin Antibody Pair Summary

Description
Quality control test: Immunoprecipitation-Western Blot (IP-WB). This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.

  • Antibody pair for IP: mouse monoclonal anti-PRF1 (300 ug)
  • Antibody pair for WB: rabbit polyclonal anti-PRF1 (50 ul)
  • Clonality
    Polyclonal
    Gene
    PRF1
    Purity
    Immunogen affinity purified

    Applications/Dilutions

    Dilutions
    • Immunoprecipitation
    • Western Blot

    Packaging, Storage & Formulations

    Storage
    Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
    Buffer
    1x PBS, pH 7.4
    Preservative
    No Preservative
    Purity
    Immunogen affinity purified

    Notes

    This product is produced by and distributed for Abnova, a company based in Taiwan.

    Alternate Names for Perforin Antibody Pair

    • Cytolysin
    • FLH2
    • HPLH2
    • HPLH2lymphocyte pore forming protein
    • Lymphocyte pore-forming protein
    • MGC65093
    • P1
    • P1PFN1
    • perforin 1 (pore forming protein)
    • Perforin
    • perforin-1
    • PFP
    • PFPcytolysin
    • PRF1

    Background

    Perforin (PRF1) is a 70 kD cytolytic protein that is the major protein constituent of cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Specifically, perforin enters the target cell's plasma membrand and creates a intercellular pore to mediate targeted cell lysis. PRF1 antibodies are useful tools for lysis research on both CTLs and NK cells.

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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    H00005551-PW2
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    Applications: WB, IP

    Publications for Perforin Antibody Pair (H00005551-PW2) (0)

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    Product General Protocols

    Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

    Video Protocols

    WB Video Protocol

    FAQs for Perforin Antibody Pair (H00005551-PW2) (0)

    There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

    Additional Perforin Products

    Research Areas for Perforin Antibody Pair (H00005551-PW2)

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    Blogs on Perforin.

    You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
    By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

    Perforin a Protective Serial Killer
    Secretory granule-mediated cell death is one of the the key mechanisms for elimination of virus-infected and transformed target cells by cytotoxic lymphocytes. Formation of the immunological synapse between an effector and a target cell leads to exocy...  Read full blog post.

    Perforin Antibodies Reveal Links to Apoptosis and Immune Response
    Perforin, also known as the pore-forming protein, pfp, is a 70 kD cytolytic protein expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It is one of the major effector molecules used by CTL and NK cel...  Read full blog post.

    Perforin Antibodies for Detecting Immune System Diseases
     Perforin is a calcium-dependent pore forming cytolytic protein. Perforin is partially homologous to the terminal components of the membrane attack complex of complement and produces pores of up to 20nm in diameter on target membranes. Killer lymphocy...  Read full blog post.

    Perforin Antibodies Reveal Links to Apoptosis and Immune Response
    Perforin, also known as the pore-forming protein, pfp, is a 70 kD cytolytic protein expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It is one of the major effector molecules used by CTL and NK cel...  Read full blog post.

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    Bioinformatics

    Gene Symbol PRF1